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Updated: May 22, 2026
09:34
Uptake of New Lipid-coated Nanoparticles Containing Falcarindiol by Human Mesenchymal Stem Cells
Published on: February 9, 2019
Dynamic cellular uptake of mixed-monolayer protected nanoparticles
Randy P Carney1, Tamara M Carney, Marie Mueller
1Department of Materials Science and Engineering, École Polytechnique Fédérale de Lausanne (EPFL), 1015, Lausanne, Switzerland.
Biointerphases
|May 17, 2012
Summary
Gold nanoparticles with specific ligand arrangements and compositions are crucial for cellular uptake. The 66-34OT nanoparticle design enhances cellular internalization across various cell types.
Area of Science:
- Biomedical Engineering
- Materials Science
- Cell Biology
Background:
- Nanoparticles (NPs) show promise in medicine, necessitating research into their cellular interactions.
- Previous work demonstrated passive diffusion and endo-/pino-cytosis for 66-34OT NPs.
Purpose of the Study:
- To analyze nanoparticle internalization by different cell lines.
- To investigate the impact of surface ligand arrangement and composition on NP cellular uptake.
Main Methods:
- Studied four types of gold nanoparticles with varying surface structures and ligand compositions.
- Analyzed NP internalization in DC2.4, 3T3, and HeLa cells.
- Quantified intracellular fluorescence and used confocal laser scanning microscopy.
Main Results:
- Cellular internalization varied significantly based on NP ligand composition and arrangement.
- 66-34OT NPs demonstrated higher internalization rates compared to MUS-only NPs.
- Internalization was also greater than NPs lacking a detectable ligand shell or those with a reversed ligand composition (34-66OT).
Conclusions:
- Ligand arrangement and composition critically influence gold nanoparticle cellular internalization.
- The 66-34OT nanoparticle design is a promising candidate for enhanced cellular uptake.
- Understanding these interactions is vital for developing effective nanomedicine applications.
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