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Quantitation of Protein Expression and Co-localization Using Multiplexed Immuno-histochemical Staining and Multispectral Imaging
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Microscopic diffusivity compartmentation in formalin-fixed prostate tissue.

Roger M Bourne1, Nyoman Kurniawan, Gary Cowin

  • 1Discipline of Medical Radiation Sciences, Faculty of Health Sciences, University of Sydney, Lidcombe 1825, Australia. roger.bourne@sydney.edu.au

Magnetic Resonance in Medicine
|July 19, 2012
PubMed
Summary
This summary is machine-generated.

This study used high-resolution MRI to analyze water diffusion in prostate tissue compartments. Findings reveal how changes in epithelial, stromal, and ductal tissue volumes correlate with prostate cancer grade.

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Area of Science:

  • Biomedical Imaging
  • Prostate Cancer Research
  • Diffusion MRI

Background:

  • Prostate cancer grading (Gleason score) is crucial for treatment decisions.
  • In vivo diffusion MRI shows a negative correlation with Gleason grade, but the underlying tissue changes are not fully understood.
  • Understanding tissue compartment diffusion is key to interpreting in vivo MRI findings.

Purpose of the Study:

  • To investigate water diffusion compartmentation in formalin-fixed prostate tissue using high-resolution MRI.
  • To correlate diffusion properties and partial volumes of epithelial, stromal, and ductal/acinar compartments with prostate cancer.
  • To explain the observed in vivo diffusion MRI-Gleason grade correlation.

Main Methods:

  • Utilized 16.4 T MR microimaging with 40-μm isotropic voxels on ten formalin-fixed prostate tissue samples.
  • Estimated mean diffusivity using manual region of interest (ROI) selection and Gaussian fitting of voxel diffusivity histograms.
  • Quantified partial volumes of epithelial, stromal, and ductal/acinar compartments.

Main Results:

  • Significantly different mean diffusivities were found across epithelial, stromal, and ductal compartments (P < 0.001) by both ROI and histogram methods.
  • Partial volumes of all three compartments differed significantly (P < 0.02) between normal and cancerous prostate tissue.
  • Epithelial tissue partial volume increased, while stromal and ductal space decreased in cancer tissue.

Conclusions:

  • The study elucidates the microstructural basis for in vivo diffusion MRI findings in prostate cancer.
  • Increased epithelial tissue volume and decreased stromal/ductal space in tumors explain the negative correlation between ADC and Gleason grade.
  • High-resolution diffusion MRI of fixed tissues provides valuable insights into cancer-related tissue alterations.