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Cloning and expression of human neuron-specific enolase cDNA in Escherichia coli
K A Pavlov1, O I Gurina, O M Antonova
1Laboratory of Immunochemistry, Department of Biological Psychiatry, V. P. Serbskii Center of Social and Forensic Psychiatry, Russian State Medical University, Moscow, Russia. pkanew@mail.ru
Abstract:
cDNA fragment encoding neuron-specific enolase was amplified from the cDNA library of human brain. Then the fragment was cloned for expression in E. coli using the vector pET28-a. High level of neuron-specific enolase expression was confirmed by SDS-PAAG electrophoresis and immunochemical identity by immunoblot analysis. The constructed producer strain is the cheapest source of neuron-specific enolase suitable for the use in diagnostic applications.
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