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G2-seq: A High Throughput Sequencing-based Technique for Identifying Late Replicating Regions of the Genome
Published on: March 22, 2018
SeqTrace: a graphical tool for rapidly processing DNA sequencing chromatograms.
1Department of Ecology and Evolutionary Biology, University of Colorado, Boulder, Colorado 80309, USA. stuckyb@colorado.edu
Journal of Biomolecular Techniques : JBT
|September 4, 2012
Summary
SeqTrace is a new, free software that automates Sanger DNA sequencing analysis. It processes chromatogram files in batches, generating high-quality DNA sequences for research.
Area of Science:
- Bioinformatics
- Molecular Biology
- Genomics
Background:
- Sanger DNA sequencing generates chromatogram trace files crucial for downstream analyses.
- Existing software solutions for processing these files are often proprietary or limited.
- Efficient batch processing of numerous trace files is essential for modern genomics research.
Purpose of the Study:
- To introduce SeqTrace, a novel, free, and open-source software application.
- To provide an automated workflow for processing Sanger DNA sequencing chromatogram files.
- To facilitate high-quality DNA sequence generation from trace data.
Main Methods:
- SeqTrace automates the identification and alignment of forward and reverse traces.
- It computes consensus sequences and filters low-quality base calls.
- The software includes a graphical interface with a chromatogram viewer and sequence editor.
Main Results:
- SeqTrace successfully automates the batch processing of large numbers of chromatogram files.
- It generates high-quality DNA sequences by identifying, aligning, and filtering trace data.
- The software provides a user-friendly interface for chromatogram visualization and sequence editing.
Conclusions:
- SeqTrace offers a robust, free, and open-source solution for Sanger DNA sequencing data analysis.
- The software streamlines the conversion of chromatogram files into usable DNA sequences.
- SeqTrace is a valuable tool for researchers requiring efficient and automated sequence data processing.
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