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Updated: May 18, 2026

Immuno-fluorescent Labeling of Microtubules and Centrosomal Proteins in Ex Vivo Intestinal Tissue and 3D In Vitro Intestinal Organoids
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Cell confinement controls centrosome positioning and lumen initiation during epithelial morphogenesis.

Alejo E Rodríguez-Fraticelli1, Muriel Auzan, Miguel A Alonso

  • 1Centro de Biología Molecular Severo Ochoa, Consejo Superior de Investigaciones Científicas-Universidad Autónoma de Madrid, 28049 Madrid, Spain.

The Journal of Cell Biology
|September 12, 2012
PubMed
Summary

Cell confinement guides epithelial organ development by controlling cell shape and promoting lumen formation. This finding reveals how physical forces impact tissue morphogenesis and could inform cancer research.

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