Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

IR Spectrometers

IR Spectrometers

There are two main infrared (IR) spectrophotometers: dispersive IR spectrometers and Fourier transform infrared (FTIR) spectrometers. In a dispersive IR spectrometer, a beam of infrared radiation produced by a hot wire is divided into two parallel equal-intensity beams using mirrors. One beam passes through the sample, while another is a reference beam. The beams then move through the monochromator, which separates the radiations into a continuous spectrum of different frequencies. The...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Refractive Index-Correlated Pseudocoloring for Adaptive Color Fusion in Holotomographic Cytology.

Cytometry. Part A : the journal of the International Society for Analytical Cytology·2026

Same author

Clinical Outcomes and Applicability of Emergency Department Termination-of-Resuscitation Rules in Super-Elderly Patients with Out-of-Hospital Cardiac Arrest: A Multicenter Analysis.

Diagnostics (Basel, Switzerland)·2026

Same author

Morphology-Preserving Holotomography: Quantitative Analysis of 3D Organoid Dynamics.

IEEE transactions on medical imaging·2026

Same author

Label-free quantitative 3D mapping of collagen architecture by holotomography.

bioRxiv : the preprint server for biology·2026

Same author

Deep Learning-Guided Holotomography Reveals Early Structural Remodelling During Pluripotency Exit.

bioRxiv : the preprint server for biology·2026

Same author

Cells Dynamically Adapt Their Nuclear Volumes and Proliferation Rates During Single to Multicellular Transitions.

Advanced science (Weinheim, Baden-Wurttemberg, Germany)·2026

Same journal

Gaussian-modulated continuous-variable quantum key distribution over 60 km fiber using an integrated silicon photonic receiver.

Optics letters·2026

Same journal

E2E-OCT: end-to-end joint learning model using optical coherence tomography images for vocal cord leukoplakia diagnosis.

Optics letters·2026

Same journal

Holographic generation of panoramic 3D scenes by concave ellipsoidal mirror reflection.

Optics letters·2026

Same journal

Dual-pilot phase recovery with pair-wise maximum-ratio combining for coherent PONs.

Optics letters·2026

Same journal

Mapping the whispering gallery modes of a CaF<sub>2</sub> disk resonator with half-tapered fibers to estimate the fundamental mode volume.

Optics letters·2026

Same journal

Quantitative estimation of deep-subwavelength scale via dark-field scattering axial energy concentration decay profiles.

Optics letters·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 18, 2026

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Published on: February 8, 2014

Fourier transform light scattering angular spectroscopy using digital inline holography.

Kyoohyun Kim¹, YongKeun Park

¹Department of Physics, Korea Advanced Institutes of Science and Technology (KAIST), Daejeon 305701, Korea.

|October 3, 2012

Summary

This summary is machine-generated.

A new method uses inline holography and Fourier transforms to measure angle-resolved light scattering (ARLS) from single particles. This technique accurately determines particle properties like refractive index and size, even for biological cells.

More Related Videos

Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy (DHM)

Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy (DHM)

Published on: November 1, 2017

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

Related Experiment Videos

Last Updated: May 18, 2026

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Digital Inline Holographic Microscopy (DIHM) of Weakly-scattering Subjects

Published on: February 8, 2014

Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy (DHM)

Quantifying Microorganisms at Low Concentrations Using Digital Holographic Microscopy (DHM)

Published on: November 1, 2017

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Compact Lens-less Digital Holographic Microscope for MEMS Inspection and Characterization

Published on: July 5, 2016

Area of Science:

Optics and Photonics
Biophysics
Materials Science

Background:

Characterizing individual micro- and nanoparticles is crucial for various scientific fields.
Existing light scattering techniques often require specialized equipment or complex sample preparation.
Accurate measurement of static and dynamic light scattering provides valuable information about particle size, refractive index, and morphology.

Purpose of the Study:

To develop a simple and practical method for measuring angle-resolved light scattering (ARLS) from individual objects.
To demonstrate the quantitative retrieval of static and dynamic scattering patterns from micrometer-sized objects.
To adapt existing microscopy setups for precise ARLS measurements.

Main Methods:

Utilized inline holography combined with Fourier transform light scattering.
Applied the technique to measure light scattering from individual polystyrene beads.
Demonstrated measurements on intact human red blood cells.

Main Results:

Successfully retrieved the refractive index and diameter of individual polystyrene beads.
Obtained quantitative static and dynamic light scattering patterns from single beads and red blood cells.
Validated the method's effectiveness for characterizing micrometer-sized objects.

Conclusions:

The developed method provides a simple, practical, and quantitative approach for ARLS measurements.
An existing microscope can be readily converted into a precise instrument for ARLS analysis.
This technique offers a versatile tool for characterizing individual particles, including biological cells.