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Cooperation between platelets and neutrophils for paf-acether (platelet-activating factor) formation
E Coëffier1, D Delautier, J P Le Couedic
1Inserm U 200, Université Paris-Sud, Clamart, France.
Journal of Leukocyte Biology
|March 1, 1990
Summary
Platelets and neutrophils cooperate to produce more platelet-activating factor (PAF-acether), a key mediator in thrombosis and inflammation. This interaction is primarily driven by a soluble factor from platelets, likely lyso-PAF, amplifying inflammatory responses.
Area of Science:
- Immunology
- Hematology
- Cellular Biology
Background:
- Platelets and neutrophils commonly associate in thrombi and inflammatory sites.
- These cell interactions contribute to the production of inflammatory mediators like hydrogen peroxides and leukotrienes.
- Platelet-activating factor (PAF-acether) is a significant mediator of thrombosis and inflammation, synthesized by both activated platelets and neutrophils.
Purpose of the Study:
- To investigate the cooperative role of platelets and neutrophils in the biosynthesis of platelet-activating factor (PAF-acether).
- To determine the contribution of platelet-derived factors, specifically lyso-PAF, in this cooperative process.
Main Methods:
- Purified human neutrophils and washed human platelets were stimulated with specific agonists (opsonized zymosan for neutrophils, thrombin for platelets).
- PAF-acether production was measured in co-cultures of platelets and neutrophils, as well as in cultures of each cell type separately.
- The effect of platelet-derived supernatants and purified lyso-PAF on neutrophil PAF-acether formation was assessed.
Main Results:
- Co-stimulation of platelets and neutrophils resulted in significantly higher PAF-acether formation (over twofold increase) compared to individual cell stimulation.
- Neutrophil PAF-acether formation was markedly enhanced when incubated with supernatants from thrombin-stimulated platelets or with synthetic lyso-PAF.
- Purified lyso-PAF from platelets significantly increased PAF-acether biosynthesis by neutrophils, indicating its crucial role in the cooperation.
Conclusions:
- A cooperative mechanism exists between platelets and neutrophils for enhanced PAF-acether formation.
- Platelet-derived lyso-PAF is identified as a key soluble factor mediating this cooperation.
- This cell-cell interaction amplifies inflammatory reactions, highlighting a molecular basis for potent inflammatory responses.