Related Experiment Video
Updated: May 17, 2026
04:14
In vivo Characterization of Endocrine Disrupting Chemical Effects via Thyroid Hormone Action Indicator Mouse
Published on: October 6, 2023
Modification of thyroid stimulating hormone estimation method in alpha prime LS system (SFRI)
1Chief Biochemist, Peerless Hospital & B.K. Roy Research Centre, 700094 Kolkata.
Indian Journal of Clinical Biochemistry : IJCB
|October 30, 2012
Summary
The alpha prime LS (APLS) system allows modifications for enzyme linked immunosorbant assay (ELISA) and chemiluminescence immunoassay (CLIA). Modifications were successfully applied to thyroid stimulating hormone (TSH) assays for improved performance.
Area of Science:
- Clinical chemistry
- Immunoassay technology
- Laboratory automation
Background:
- The alpha prime LS (APLS) system integrates both ELISA and CLIA platforms.
- Programmable features allow for system customization.
- Standard protocols may require optimization for specific assay kits.
Purpose of the Study:
- To describe modifications made to the APLS system for routine immunoassays.
- To address performance issues encountered with specific assay kits.
Main Methods:
- The study focused on the APLS system, utilizing both ELISA and CLIA capabilities.
- User-defined program modifications were implemented.
- Specific adjustments included incubation temperature, decontamination time, signal reagent usage, and calibration patterns.
- Troubleshooting was performed on thyroid stimulating hormone (TSH) Monobind kits.
Main Results:
- Successful modifications were made to the APLS system's assay parameters.
- The implemented changes resolved issues encountered during routine TSH testing.
- Optimized protocols enhanced the performance of specific immunoassay kits.
Conclusions:
- The APLS system's flexibility allows for effective troubleshooting and optimization of immunoassay protocols.
- User-defined programming is a valuable tool for adapting the system to specific diagnostic needs.
- Customization of parameters like temperature and calibration is crucial for reliable TSH testing.
Related Concept Videos
01:20Synthesis and Regulation of Thyroid Hormones
Low blood levels of the thyroid hormones — triiodothyronine (T3) and thyroxine (T4) — signal the hypothalamus to release the thyrotropin-releasing hormone (TRH). TRH then reaches the pituitary gland and stimulates the release of thyroid-stimulating hormone(TSH) into the bloodstream.
Upon reaching the thyroid gland, TSH stimulates the follicular cells' active uptake of iodide ions from the blood. The ions diffuse to the apical surface of the cells and are oxidized to iodine. The iodine is then...
Upon reaching the thyroid gland, TSH stimulates the follicular cells' active uptake of iodide ions from the blood. The ions diffuse to the apical surface of the cells and are oxidized to iodine. The iodine is then...
01:25Hyperthyroidism I: Introduction
Hyperthyroidism is a type of thyrotoxicosis characterized by the thyroid gland's overproduction of the thyroid hormones triiodothyronine (T3) and thyroxine (T4). This hormone excess increases the basal metabolic rate and enhances sensitivity to catecholamines.DiagnosisDiagnosis is based on clinical features and biochemical testing. It typically shows suppressed thyroid-stimulating hormone (TSH) levels below 0.4 mIU/L, with elevated free T3 and/or T4. Additional tests, including thyroid...
01:26Therapeutic Drug Monitoring: Drug Analysis Methods
Therapeutic Drug Monitoring (TDM) is a clinical practice that measures specific drug levels in a patient's blood or body tissues to tailor drug therapy effectively. This monitoring is critical for managing drugs with narrow therapeutic indices like digoxin and phenytoin, ensuring they are both safe and effective. For instance, monitoring theophylline levels in asthma patients involves precision and sensitivity to adjust doses according to individual responses to therapy, ensuring efficacy and...