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High-throughput RNA in situ hybridization in mouse retina

Seth Blackshaw1

  • 1The Salomon H. Snyder Department of Neuroscience, John Hopkins University School of Medicine, Baltimore, MD, USA. sblack@jhmi.edu

Methods in Molecular Biology (Clifton, N.J.)
|November 15, 2012
PubMed

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In-situ Hybridization02:31

In-situ Hybridization

In situ hybridization (ISH) is a technique used to detect and localize specific DNA or RNA molecules in cells, tissue, or tissue sections using a labeled probe. The technique was first used in 1969 for the investigation of nucleic acids. It is currently an essential tool in scientific research and clinical settings, especially for diagnostic purposes.
Types of probes and labels
A probe is a complementary strand of DNA or RNA that binds to corresponding nucleotide sequences in a cell. Many...