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Temperature- and salinity-decoupled overproduction of hydroxyectoine by Chromohalobacter salexigens
Javier Rodríguez-Moya1, Montserrat Argandoña, Fernando Iglesias-Guerra
1Department of Microbiology and Parasitology, Faculty of Pharmacy, University of Seville, Seville, Spain.
Abstract:
Hydroxyectoine overproduction by the natural producer Chromohalobacter salexigens is presented in this study. Genetically engineered strains were constructed that at low salinity coexpressed, in a vector derived from a native plasmid, the ectoine (ectABC) and hydroxyectoine (ectD) genes under the control of the ectA promoter, in a temperature-independent manner. Hydroxyectoine production was further improved by increasing the copies of ectD and using a C. salexigens genetic background unable to synthesize ectoines.
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