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Stem cell identification by DyeCycle Violet side population analysis.

William G Telford1

  • 1Experimental Transplantation and Immunology Branch, National Institutes of Health, Bethesda, MD, USA. telfordw@mail.nih.gov

Methods in Molecular Biology (Clifton, N.J.)
|November 27, 2012
PubMed
Summary
This summary is machine-generated.

Side population (SP) analysis using DyeCycle Violet (DCV) offers a method for identifying stem cells and cancer stem cells. This chapter details DCV SP analysis procedures, including immunolabeling and data analysis.

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Area of Science:

  • Biotechnology
  • Cell Biology
  • Hematology

Background:

  • Side population (SP) analysis is crucial for identifying stem and progenitor cells.
  • Hoechst 33342 is a traditional dye for SP analysis.
  • Cancer stem cells can also be identified using SP analysis.

Purpose of the Study:

  • To review the history and provide a detailed procedure for DyeCycle Violet (DCV) SP analysis.
  • To cover critical parameters for optimal DCV labeling.
  • To detail the integration of immunolabeling with DCV SP analysis.

Main Methods:

  • Utilizing DyeCycle Violet (DCV), a DNA-binding dye with a violet excitation spectrum.
  • Performing SP analysis on flow cytometers equipped with violet laser diodes.
  • Integrating simultaneous immunolabeling with DCV SP analysis.

Main Results:

  • DCV provides an alternative to Hoechst 33342 for SP analysis.
  • Detailed procedures for labeling, data acquisition, and analysis are provided.
  • Successful integration of immunolabeling with DCV SP analysis is demonstrated.

Conclusions:

  • DCV SP analysis is a valuable technique for stem cell identification.
  • The chapter provides a comprehensive guide for implementing DCV SP analysis.
  • This method aids in the study of hematopoietic and non-hematopoietic stem cells and cancer stem cells.