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Related Concept Videos

Super-resolution Fluorescence Microscopy01:37

Super-resolution Fluorescence Microscopy

Super-resolution fluorescence microscopy (SRFM) provides a better resolution than conventional fluorescence microscopy by reducing the point spread function (PSF). PSF is the light intensity distribution from a point that causes it to appear blurred. Due to PSF, each fluorescing point appears bigger than its actual size, and it is the PSF interference of nearby fluorophores that causes the blurred image. Various approaches to achieving higher resolution through SRFM have recently been developed.
Fluorescence and Phosphorescence: Instrumentation01:25

Fluorescence and Phosphorescence: Instrumentation

Fluorometers and spectrofluorometers are two types of instruments used for measuring molecular fluorescence. These instruments differ in how they select excitation and emission wavelengths and the type of light sources they utilize. Fluorometers use absorption interference filters to choose excitation and emission wavelengths. The excitation source in a fluorometer is typically a low-pressure mercury vapor lamp that emits intense lines distributed throughout the ultraviolet and visible regions.
Protein Dynamics in Living Cells01:19

Protein Dynamics in Living Cells

Different fluorescence-based techniques are used to study the protein dynamics in living cells. These techniques include FRAP, FRET, and PET.
Fluorescent recovery after photobleaching (FRAP) is a fluorescent-protein-based detection technique used to quantify protein movement rates within the cell. This method exposes a small portion of the cell to an intense laser beam. The laser beam causes permanent photobleaching of the fluorophore-tagged proteins in the exposed region. As the bleached...

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Updated: May 16, 2026

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
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Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells

Published on: December 11, 2021

Dispersion-relation fluorescence spectroscopy.

Ru Wang1, Lei Lei, Yingxiao Wang

  • 1Quantitative Light Imaging Laboratory, Department of Mechanical Science and Engineering, Beckman Institute for Advanced Science and Technology, University of Illinois at Urbana-Champaign, Urbana, Illinois 61801, USA.

Physical Review Letters
|December 11, 2012
PubMed
Summary
This summary is machine-generated.

Dispersion-relation fluorescence spectroscopy (DFS) offers a novel method to study molecular transport dynamics across various scales. This technique accurately quantifies diffusion and advection, distinguishing active transport in living cells like the actin cytoskeleton.

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Last Updated: May 16, 2026

Dual-Color Fluorescence Cross-Correlation Spectroscopy to Study Protein-Protein Interaction and Protein Dynamics in Live Cells
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Measuring Diffusion Coefficients via Two-photon Fluorescence Recovery After Photobleaching

Published on: February 26, 2010

Area of Science:

  • Biophysics
  • Cell Biology
  • Microscopy Techniques

Background:

  • Fluorescence microscopy is crucial for live-cell dynamics and function studies.
  • Fluorescence correlation spectroscopy analyzes molecular transport but is limited to fixed spatial scales.

Purpose of the Study:

  • To introduce a new method, dispersion-relation fluorescence spectroscopy (DFS), for studying transport dynamics over broad spatial and temporal scales.
  • To enable quantitative analysis of molecular motion, including diffusion and advection, in biological systems.

Main Methods:

  • Utilizing fluorophore-labeled molecules to generate spontaneous fluorescence intensity fluctuations.
  • Analyzing these fluctuations via the dispersion-relation fluorescence spectroscopy (DFS) approach to quantify mass transport dynamics.
  • Characterizing transport data by the effective dispersion relation.

Main Results:

  • DFS successfully distinguished between diffusive and advection motion in a model system.
  • Quantitatively accurate values for diffusivities and advection velocities were obtained.
  • DFS demonstrated the ability to differentiate directed from diffusive transport in living cells, observing active transport in the actin cytoskeleton parallel to fibers and diffusive motion perpendicularly.

Conclusions:

  • Dispersion-relation fluorescence spectroscopy (DFS) is a powerful new technique for analyzing molecular transport dynamics.
  • DFS provides spatially resolved information, enabling the distinction between active and diffusive transport mechanisms in complex biological environments.
  • The method accurately quantifies transport parameters, offering insights into cellular processes like cytoskeletal dynamics.