Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

DNA Packaging

DNA Packaging

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Influence of different layout of rigid and flexible structures on methane explosion in oil and gas gathering and transportation station.

PloS one·2026

Same author

Complex cooperativity in DNA origami revealed via design-dependent defectivity.

Nucleic acids research·2026

Same author

Acute Epstein-Barr Virus-Related Myopericarditis in an Immunocompetent Young Adult.

Cureus·2025

Same author

Variable gain DNA nanostructure charge amplifiers for biosensing.

Nanoscale·2024

Same author

DNA Origami Design: A How-To Tutorial.

Journal of research of the National Institute of Standards and Technology·2024

Same author

DNA-PAINT Probe Modifications Support High-Resolution Imaging with Shorter Binding Domains.

ACS nano·2024

Same journal

An intrinsically stretchable nanowire-based sensing patch for wearable analysis of sweat chloride ion composition.

Chemical communications (Cambridge, England)·2026

Same journal

A sterically rigid-flexible balanced NHC-Pd precatalyst for room-temperature solvent-free C-N coupling of benzocyclic amines.

Chemical communications (Cambridge, England)·2026

Same journal

Portable fluorescent conjugated microporous polymer sensor coupled with a smartphone for on-site Fe<sup>3+</sup> detection in water.

Chemical communications (Cambridge, England)·2026

Same journal

Accelerated discovery of NO<sub>3</sub>RR single-atom catalysts <i>via</i> high-throughput DFT and machine learning.

Chemical communications (Cambridge, England)·2026

Same journal

Wafer-scale robust graphene electronics under industrial processing conditions.

Chemical communications (Cambridge, England)·2026

Same journal

Subnanoscale IrW oxide anodes: breaking immiscibility for high activity and durability in water electrolysis.

Chemical communications (Cambridge, England)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 15, 2026

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

Published on: August 26, 2009

Quantum dot-DNA origami binding: a single particle, 3D, real-time tracking study.

Kan Du¹, Seung Hyeon Ko, Gregg M Gallatin

¹Center for Nanoscale Science and Technology, National Institute of Standards and Technology, Gaithersburg, MD 20899, USA.

Chemical Communications (Cambridge, England)

|December 19, 2012

Summary

This summary is machine-generated.

Researchers directly observed quantum dot-DNA origami binding events in real-time. This method precisely distinguished between free quantum dots and their conjugates using diffusion and photon correlation measurements.

More Related Videos

Visualizing the Interaction Between the Qdot-labeled Protein and Site-specifically Modified λ DNA at the Single Molecule Level

Visualizing the Interaction Between the Qdot-labeled Protein and Site-specifically Modified λ DNA at the Single Molecule Level

Published on: July 17, 2018

A Protocol for Real-time 3D Single Particle Tracking

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

Related Experiment Videos

Last Updated: May 15, 2026

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

Combining QD-FRET and Microfluidics to Monitor DNA Nanocomplex Self-Assembly in Real-Time

Published on: August 26, 2009

Visualizing the Interaction Between the Qdot-labeled Protein and Site-specifically Modified λ DNA at the Single Molecule Level

Visualizing the Interaction Between the Qdot-labeled Protein and Site-specifically Modified λ DNA at the Single Molecule Level

Published on: July 17, 2018

A Protocol for Real-time 3D Single Particle Tracking

A Protocol for Real-time 3D Single Particle Tracking

Published on: January 3, 2018

Area of Science:

Nanotechnology
Biophysics
Molecular Biology

Background:

Quantum dots (QDs) and DNA origami are advanced nanomaterials with diverse applications.
Understanding the binding kinetics of QD-DNA origami conjugates is crucial for developing nanodevices.
Real-time single-molecule analysis provides detailed insights into molecular interactions.

Purpose of the Study:

To directly observe and characterize the binding process between quantum dots and DNA origami at the single-molecule level.
To develop a method for distinguishing free quantum dots from QD-DNA origami conjugates.
To utilize advanced tracking and correlation techniques for precise kinetic measurements.

Main Methods:

Employed a 3D, real-time, single-particle tracking system to monitor binding events.
Measured the diffusion coefficient of individual particles.
Analyzed the second-order photon correlation function, g(2)(τ), of the detected signals.

Main Results:

Successfully observed single-molecule binding events between quantum dots and DNA origami in real-time.
Demonstrated the ability to differentiate free quantum dots from various nQdot-origami conjugates.
Precise measurements of diffusion coefficients and g(2)(τ) confirmed the distinction between different molecular states.

Conclusions:

The developed single-particle tracking method enables direct observation of QD-DNA origami binding.
This technique effectively distinguishes between free QDs and their conjugates based on their dynamic and optical properties.
Provides a robust platform for studying nanoparticle-nucleic acid interactions in nanotechnology and molecular assembly.