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Related Concept Videos

Hyperthyroidism II: Pathophysiology01:27

Hyperthyroidism II: Pathophysiology

Hyperthyroidism is a hypermetabolic state caused by elevated levels of thyroid hormones, triiodothyronine (T3) and thyroxine (T4). It results from dysregulation at the thyroid, pituitary, or immune system level and affects multiple organ systems.PathophysiologyThe most common cause of hyperthyroidism is Graves’ disease, an autoimmune disorder in which antibodies, specifically thyroid-stimulating antibodies (TSAb), a subtype of TSH receptor antibodies (TRAb), bind to and activate TSH receptors...
Synthesis and Regulation of Thyroid Hormones01:20

Synthesis and Regulation of Thyroid Hormones

Low blood levels of the thyroid hormones — triiodothyronine (T3) and thyroxine (T4) — signal the hypothalamus to release the thyrotropin-releasing hormone (TRH). TRH then reaches the pituitary gland and stimulates the release of thyroid-stimulating hormone(TSH) into the bloodstream.
Upon reaching the thyroid gland, TSH stimulates the follicular cells' active uptake of iodide ions from the blood. The ions diffuse to the apical surface of the cells and are oxidized to iodine. The iodine is then...
Atomic Absorption Spectroscopy: Interference01:25

Atomic Absorption Spectroscopy: Interference

Interference leads to systematic error in atomic absorption (AA) measurements by enhancing or diminishing the analytical signal or the background. These interferences can be grouped into three main categories: spectral interference, chemical interference, and physical interference.
Spectral interference occurs when signals from other elements or molecules overlap with the analyte signal, falsely elevating or masking the analyte's absorbance. This interference can be corrected using Zeeman,...
Hyperthyroidism I: Introduction01:25

Hyperthyroidism I: Introduction

Hyperthyroidism is a type of thyrotoxicosis characterized by the thyroid gland's overproduction of the thyroid hormones triiodothyronine (T3) and thyroxine (T4). This hormone excess increases the basal metabolic rate and enhances sensitivity to catecholamines.DiagnosisDiagnosis is based on clinical features and biochemical testing. It typically shows suppressed thyroid-stimulating hormone (TSH) levels below 0.4 mIU/L, with elevated free T3 and/or T4. Additional tests, including thyroid...
Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences01:20

Inductively Coupled Plasma-Mass Spectrometry (ICP-MS): Interferences

Inductively coupled plasma–mass spectrometry (ICP–MS) is a highly selective and sensitive technique for accurate elemental analysis. Though the analysis of ICP–MS mass spectra is comparatively straightforward, it is affected by spectroscopic and non-spectroscopic interferences. Spectroscopic interferences arise when the plasma contains ionic species with an m/z value the same as the analyte ion. Spectroscopic interference can be categorized as isobaric, polyatomic ions, and refractory oxide ion...

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Generation of a Mouse Spontaneous Autoimmune Thyroiditis Model
04:39

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Published on: March 17, 2023

TSH-assay interference: still with us.

Laurence Verdickt1, Dominique Maiter, Louis Depraetere

  • 1Department of Laboratory Medicine, Cliniques Universitaires St-Luc and Université Catholique de Louvain, Brussels, Belgium.

Clinical Laboratory
|January 8, 2013
PubMed
Summary
This summary is machine-generated.

Heterophilic antibodies can interfere with thyroid stimulating hormone (TSH) tests, causing falsely high results. Different automated platforms may show varying susceptibility to such interferences, impacting thyroid function diagnosis.

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Area of Science:

  • Clinical chemistry
  • Immunology
  • Endocrinology

Background:

  • Thyroid stimulating hormone (TSH) measurement is crucial for evaluating thyroid function.
  • Assay interference can lead to inaccurate TSH levels, resulting in unnecessary investigations and misdiagnosis.

Observation:

  • A case report details interference by heterophilic antibodies in an automated TSH assay, yielding false-positive results.
  • This specific interference was not observed on a different automated analytical platform.

Findings:

  • The susceptibility to heterophilic antibody interference varies between automated immunoassay platforms.
  • Factors such as assay design, antibody selection, and reagent volumes influence interference sensitivity.

Implications:

  • Accurate detection and reporting of immunoassay interferences are vital for improving diagnostic test performance.
  • Multidisciplinary collaboration between clinicians and laboratory professionals is essential for effective interference tracking and management.