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Related Experiment Videos

A fast responding fibre optic glucose biosensor based on an oxygen optrode.

B P Schaffar1, O S Wolfbeis

  • 1Department of Enzyme Technology, GBF, Braunschweig, FRG.

Biosensors & Bioelectronics
|January 1, 1990
PubMed
Summary

This study presents a rapid glucose biosensor using an oxygen optrode and immobilized glucose oxidase (GOD). It offers fast response times and stable performance for continuous glucose monitoring.

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Area of Science:

  • Biomedical Engineering
  • Analytical Chemistry

Background:

  • Continuous glucose monitoring is crucial for diabetes management.
  • Existing biosensors face challenges with response time and stability.
  • Oxygen optrode-based biosensors offer a promising alternative detection method.

Purpose of the Study:

  • To develop and characterize a fast-responding glucose biosensor.
  • To investigate the impact of glucose oxidase (GOD) quality on sensor performance.
  • To enhance the analytical range of the biosensor for broader clinical application.

Main Methods:

  • Immobilization of glucose oxidase (GOD) onto an oxygen optrode using carbon black and glutardialdehyde.
  • Measurement of oxygen consumption via fluorescence quenching in a flow-through cell.

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  • Evaluation of sensor response time, linear analytical range, and long-term stability.
  • Development of a device to extend the glucose concentration range.

    • Main Results:

    • Response times (tau 90) ranged from 8-60 seconds.
    • Linear analytical range was observed from 0.01 to 2 mM glucose.
    • Long-term stability (tau 1/2) varied from 1 to 20 weeks depending on GOD quality.
    • An extended analytical range up to 200 mM glucose was achieved with a simple device.

    Conclusions:

    • The developed oxygen optrode-based glucose biosensor demonstrates rapid response and good stability.
    • Different GOD qualities significantly influence sensor performance metrics.
    • The biosensor shows potential for continuous glucose monitoring applications.
    • The device effectively expands the analytical range for higher glucose concentrations.