Related Experiment Video
Updated: May 14, 2026
08:06
Porous Substrate-Based Electroporation with Transepithelial Electrical Impedance Monitoring
Published on: September 27, 2024
On-chip electroporation: characterization, modeling and experimental results.
Ebrahim Ghafar-Zadeh1, Bahareh Gholamzadeh, Falah Awwad
1Department of Electrical Engineering, Ecole Polytechnique de Montréal. ebrahim.ghafar-zadeh@polymtl.ca
Summary
This study introduces an on-chip electroporation (EP) technique for precise nano-injection of biomolecules into single cells. Preliminary experiments show its potential for advanced cellular research.
Area of Science:
- Biotechnology
- Cell Biology
- Electrical Engineering
Background:
- Electroporation (EP) is a method utilizing electrical stimulation to create temporary nanopores in cell membranes.
- Precise delivery of biomolecules into single cells is crucial for various biological and medical applications.
Purpose of the Study:
- To describe an on-chip electroporation (EP) method for high-precision nano-injection of biomolecules into single cells.
- To present computational models of cultured cells and microelectrodes for this technique.
Main Methods:
- Development of an on-chip electroporation system.
- Computational modeling of cell-microelectrode interactions.
- Experimental validation using mouse fibroblast cells, low-frequency electrical fields (10 Hz), and propidium iodide (PI).
Main Results:
- Demonstration of preliminary experimental results for the on-chip EP method.
- Culturing of mouse fibroblast cells on microelectrodes subjected to an electrical field.
- Successful application of the method in the presence of propidium iodide.
Conclusions:
- The developed on-chip EP method offers a promising approach for high-precision nano-injection into single cells.
- Computational models and experimental validation provide a foundation for further refinement and application of the technique.
