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Published on: August 25, 2018
Effects of strigolactone-biosynthesis inhibitor TIS108 on Arabidopsis
Shinsaku Ito1, Mikihisa Umehara, Atsushi Hanada
1Department of Applied Biological Chemistry; The University of Tokyo; Tokyo, Japan; Department of Bioscience; Faculty of Applied Bioscience; Tokyo University of Agriculture; Tokyo, Japan.
Abstract:
TIS108 is a triazole-type strigolactone (SL)-biosynthesis inhibitor that reduces the level of 2'-epi-5-deoxystrigol (epi-5DS) in rice. Here we report the effects of TIS108 on Arabidopsis. Treatment of TIS108 increased the number of branches and repressed root hair elongation as was observed in SL-deficient mutants, and co-application of GR24, a synthetic SL analog, recovered the TIS108-induced phenotype to that of wild-type. In addition, MAX3 and MAX4 genes in the SL-biosynthesis pathway were upregulated in TIS108-treated Arabidopsis, probably due to feedback regulation caused by SL deficiency. These results indicate that TIS108 is an effective tool for regulating SL production in Arabidopsis.

