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Recombinant Protein Expression, Crystallization, and Biophysical Studies of a Bacillus-conserved Nucleotide Pyrophosphorylase, BcMazG
Published on: May 16, 2017
Molecular cloning and sequence analysis of an extracellular protease from four Bacillus subtilis strains
Xujun Han1, Yuh Shiwa, Masanori Itoh
1United Graduate School of Agricultural Science, Gifu University, Gifu, Japan.
Abstract:
Four Bacillus subtilis strains were isolated from traditional fermented foods, and the sequences of their extracellular alkaline proteases (AprE) were analyzed and cloned. The recombinant enzymes synthesized by means of Escherichia coli exhibited high proteolytic activity. AprE CN2 showed hydrolytic activity 4-fold higher than that of AprE 168. This activity was also seen in the presence of relatively high NaCl concentrations.
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