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Updated: May 12, 2026
Computed Tomography-guided Time-domain Diffuse Fluorescence Tomography in Small Animals for Localization of Cancer Biomarkers
Published on: July 17, 2012
Phantom and mouse experiments of time-domain fluorescence tomography using total light approach
Shinpei Okawa1, Akira Yano, Kazuki Uchida
1Department of Mechanical Engineering and Intelligent Systems, University of Electro-Communications, 1-5-1 Chofuga-oka, Chofu, Tokyo 182-8585, Japan ; Currently with the Department of Medical Engineering, National Defense Medical College, 3-2 Namiki, Tokorozawa, Saitama 359-8513, Japan.
Abstract:
Phantom and mouse experiments of time-domain fluorescence tomography were conducted to demonstrate the total light approach which was previously proposed by authors. The total light approach reduces the computation time to solve the forward model for light propagation. Time-resolved temporal profiles were acquired for cylindrical phantoms having single or double targets containing indocyanine green (ICG) solutions. The reconstructed images of ICG concentration reflected the true distributions of ICG concentration with a spatial resolution of about 10 mm. In vivo experiments were conducted using a mouse in which an ICG capsule was embedded beneath the skin in the abdomen. The reconstructed image of the ICG concentration again reflected the true distribution of ICG although artifacts due to autofluorescence appeared in the vicinity of the skin. The effectiveness of the total light approach was demonstrated by the phantom and mouse experiments.
