Related Experiment Video
Updated: May 12, 2026
Toxin Induction and Protein Extraction from Fusarium spp. Cultures for Proteomic Studies
Published on: February 16, 2010
Species specific profiles of secondary metabolites within the genusFusarium, obtained by reversed phase high
1Department of Biotechnology, Building 221, The Technical University of Denmark, DK-2800, Lyngby, Denmark.
Abstract:
Extracts of pure cultures ofFusarium species were analysed by reversed phase High Performance Liquid Chromatography. The peaks recorded by the Photodiode-Array UV-VIS detector were characterized by their alkylphenone retention time index, and by their UV-VIS spectrum (200-600 nm). The number of biosynthetic families (chemosyndromes) was counted by grouping the peaks having similar UV-VIS spectra.All the investigatedFusarium species had a specific profile of 4-13 chemosyndromes, of which some were species specific while others could be recorded in more than one species.
