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[HPLC fingerprint of compound xueshuantong capsule]
Jie-Ping Liang1, Zhong-Zheng Liu, Wei Peng
1School of Life Sciences, Sun Yat-sen University, Guangzhou 510275, China. liangjp2@163.com
Objective:
To establish the HPLC fingerprint of Compound Xueshuantong Capsule.
Methods:
Dionex Acclaim 120 C18 column (4.6 mm x 150 mm, 3 microm) was used with acetonitrile (A) and 0.05% phosphoric acid (B) in gradient elution mode. The elution profile was:0-50 min (15%-->34% A), 50-95 min (34%-->75% A); The detective wavelength was 203 nm and 270 nm. The column temperature was set at 25 degrees C and the flow rate was 1.0 mL/min.
Results:
In the established fingerprint,42 common peaks covering 4 medicinal materials were detected, and 23 chemical compounds were identified by RRLC/MS/MS and DAD spetra.
Conclusion:
The method can be used for quality control of Compound Xueshuantong Capsule with great precision, accuracy and good reproducibility.