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Related Experiment Video

Updated: May 11, 2026

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
06:12

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

Colorimetric surface plasmon resonance imaging (SPRI) biosensor array based on polarization orientation.

Chi Lok Wong1, George Chung Kit Chen, Xiaochao Li

  • 1Bio-optical Imaging Group, Singapore Bioimaging Consortium, Helios #02-02, 11 Biopolis Way, Singapore 138667, Singapore. dave_wong@sbic.a-star.edu.sg

Biosensors & Bioelectronics
|May 7, 2013
PubMed
Summary
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This study introduces a colorimetric surface plasmon resonance (SPR) biosensor array for enhanced bio-molecular detection. The novel sensor offers improved resolution and multiplexing capabilities for high-throughput screening and diagnostics.

Area of Science:

  • * Biophotonics and Biosensing
  • * Surface Plasmon Resonance (SPR) Imaging

Background:

  • * Existing phase-sensitive SPR imaging sensors have limitations in resolution and complexity.
  • * Multiplexed, high-throughput detection of bio-molecular interactions is crucial for diagnostics and screening.

Purpose of the Study:

  • * To develop a colorimetric SPR imaging biosensor array with enhanced resolution and simplified readout.
  • * To demonstrate the sensor's capability for real-time, simultaneous detection of bio-molecular binding events.

Main Methods:

  • * Utilized polarization orientation rotation for colorimetric SPR imaging.
  • * Measured spectral characteristic variations at surface plasmon excitation.
  • * Performed experiments detecting anti-bovine serum albumin (anti-BSA) and BSA antigen binding.

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Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)
09:35

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)

Published on: November 29, 2014

Related Experiment Videos

Last Updated: May 11, 2026

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets
06:12

Multimodal Analytical Platform on a Multiplexed Surface Plasmon Resonance Imaging Chip for the Analysis of Extracellular Vesicle Subsets

Published on: March 17, 2023

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)
09:35

Real Time Measurements of Membrane Protein:Receptor Interactions Using Surface Plasmon Resonance (SPR)

Published on: November 29, 2014

Main Results:

  • * Achieved a sensor resolution of 8.26 ng/ml (125 pM), an order of magnitude improvement over existing sensors.
  • * Demonstrated multiplexed, real-time monitoring of binding interactions on a 5x4 protein array.
  • * Enabled easy optical colorimetric image readout without complex phase modulation.

Conclusions:

  • * The colorimetric SPR imaging biosensor array offers superior resolution and simplified detection.
  • * The technology shows significant potential for high-throughput clinical diagnostics, biomarker screening, and drug discovery.