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An alternative direct compound dispensing method using the HP D300 digital dispenser.
Raisa E Jones1, Wei Zheng, John C McKew
11National Center for Advancing Translational Sciences, National Institutes of Health, Bethesda, MD, USA.
Journal of Laboratory Automation
|May 28, 2013
Summary
The HP D300 dispenser uses inkjet technology for accurate compound dispensing in drug discovery assays. This instrument streamlines workflows and improves throughput for 96- and 384-well plates, yielding reproducible results.
Area of Science:
- Biochemistry
- Molecular Biology
- Drug Discovery
Background:
- Accurate compound titration and dispensing are essential for in vitro drug discovery.
- Inkjet technology offers a novel approach to small-volume dispensing for concentration-response testing.
Purpose of the Study:
- To evaluate the performance of the HP D300 dispenser, utilizing inkjet technology, in various drug screening assays.
- To assess the accuracy, reproducibility, and user-friendliness of the D300 dispenser compared to traditional methods.
Main Methods:
- The HP D300 dispenser was evaluated using a biochemical assay, a cell-based reporter gene assay, and a cytotoxicity assay.
- Compound dispensing was performed using the direct dilution paradigm with the D300 instrument.
- Results were compared with those obtained using conventional pin tools.
Main Results:
- The HP D300 dispenser demonstrated user-friendly software and a streamlined dispensing process.
- The instrument produced clean and reproducible results, correlating well with established dispensing methods.
- A limitation identified was its current incompatibility with 1536-well high-density plates.
Conclusions:
- The HP D300 dispenser is a valuable tool for improving compound dispensing throughput in 96- and 384-well plate formats.
- Inkjet dispensing technology shows promise for drug screening and development, offering reliable performance.
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