Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Experiment Video

Updated: May 10, 2026

Construction of a High Resolution Microscope with Conventional and Holographic Optical Trapping Capabilities
09:12

Construction of a High Resolution Microscope with Conventional and Holographic Optical Trapping Capabilities

Published on: April 22, 2013

Towards Spatio-Temporal Control in Optical Trapping.

Debjit Roy1, Arijit Kumar De, Debabrata Goswami

  • 1Department of Chemistry, Indian Institute of Technology Kanpur, UP - 208016, India.

Proceedings of Spie--The International Society for Optical Engineering
|July 2, 2013
PubMed
Summary
This summary is machine-generated.

Related Concept Videos

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Linear and Nonlinear Spectroscopic Insights Into the Synergistic Solvation of Rhodamine-6G in Methanol-Dichloromethane Binary Solvent Mixture.

Journal of fluorescence·2026
Same author

Convective Onset in Thermal Lensing: Systematic Pulse-Number-Dependent Experiments and Theoretical Insights into the Femtosecond Regime.

The journal of physical chemistry. B·2026
Same author

Supramolecular Nanoassemblies Harness Radical Excited-State Cascade Electron Transfer for Selective CO<sub>2</sub> Photoreduction.

Small (Weinheim an der Bergstrasse, Germany)·2026
Same author

Femtosecond-laser-induced optical confinement with ping-pong motion.

The Journal of chemical physics·2025
Same author

Compositional Analysis of Fragrance Accords Using Femtosecond Thermal Lens Spectroscopy.

Chemistry, an Asian journal·2025
Same author

Toward measurements of absolute membrane potential in Bacillus subtilis using fluorescence lifetime.

Biophysical reports·2025
Same journal

From Geometry to Intensity: A Coarse-to-Fine Pipeline for Unsupervised 3D Ultrasound Stitching.

Proceedings of SPIE--the International Society for Optical Engineering·2026
Same journal

AVA: Automated Viewability Analysis for Ureteroscopic Intrarenal Surgery.

Proceedings of SPIE--the International Society for Optical Engineering·2026
Same journal

Kidney Endoscopy Video to Preoperative CT Alignment for Depth Estimation.

Proceedings of SPIE--the International Society for Optical Engineering·2026
Same journal

Deep learning‑based cell type prediction in lung tissue from brightfield histology using CODEX-derived labels.

Proceedings of SPIE--the International Society for Optical Engineering·2026
Same journal

Reconstructing physiological signals from fMRI across the adult lifespan.

Proceedings of SPIE--the International Society for Optical Engineering·2026
Same journal

Axially Swept Light-Sheet Microscopy using scattering and fluorescence contrast mechanisms.

Proceedings of SPIE--the International Society for Optical Engineering·2026
See all related articles

Stable 3D optical trapping of microparticles and nanoparticles was achieved using femtosecond lasers. This method enables trapping at lower powers than continuous-wave lasers, highlighting the pulse duration

Area of Science:

  • Optics and Photonics
  • Biophysics
  • Materials Science

Background:

  • Optical trapping utilizes focused laser beams to manipulate microscopic particles.
  • Continuous-wave (CW) lasers are commonly used but require higher powers for stable trapping.
  • Femtosecond lasers offer unique pulse characteristics that may enhance trapping capabilities.

Purpose of the Study:

  • To investigate stable 3-dimensional optical trapping of microspheres and nanoparticles.
  • To compare the trapping efficiency of continuous-wave (CW) versus femtosecond lasers.
  • To evaluate the impact of laser beam profile on optical trapping performance.

Main Methods:

  • Stable 3D optical trapping of 1μm polystyrene microspheres using CW and femtosecond lasers.
  • Trapping of 100nm latex nanoparticles using high repetition rate femtosecond lasers at low average power.
Keywords:
Optical trapRayleigh particlesfemto-second laserpeak powertwo-photon fluorescence

More Related Videos

Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers
09:56

Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers

Published on: August 31, 2021

Optical Trapping of Nanoparticles
13:39

Optical Trapping of Nanoparticles

Published on: January 15, 2013

Related Experiment Videos

Last Updated: May 10, 2026

Construction of a High Resolution Microscope with Conventional and Holographic Optical Trapping Capabilities
09:12

Construction of a High Resolution Microscope with Conventional and Holographic Optical Trapping Capabilities

Published on: April 22, 2013

Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers
09:56

Direct Force Measurements of Subcellular Mechanics in Confinement using Optical Tweezers

Published on: August 31, 2021

Optical Trapping of Nanoparticles
13:39

Optical Trapping of Nanoparticles

Published on: January 15, 2013

  • Visualization via dark-field microscopy and diagnostics using noise-free two-photon fluorescence.
  • Comparison of Gaussian and flat-top Gaussian beam profiles for optical trapping.

    • Main Results:

    • Stable 3D trapping of 1μm polystyrene microspheres was achieved with both CW and femtosecond lasers.
    • 100nm latex nanoparticles were stably trapped using femtosecond lasers at power levels insufficient for CW trapping.
    • Two-photon fluorescence provided noise-free, 3D resolved diagnostics of trapped particles.
    • Laser spatial mode significantly influences optical trapping efficiency, with flat-top Gaussian beams showing distinct effects.

    Conclusions:

    • Femtosecond lasers enable stable optical trapping of nanoparticles at significantly lower average powers than CW lasers.
    • The temporal characteristics of femtosecond pulses are crucial for trapping smaller nanoparticles.
    • Laser beam spatial mode is a critical parameter for optimizing optical trapping.