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Does centrifugation and semen processing with swim up at 37°C yield sperm with better DNA integrity compared to
Deepthi Repalle1, Priya Bhave Chittawar, Shilpa Bhandari
1Department of Reproductive Medicine, Mohak Hi-tech Speciality Laparoscopy and Infertility Centre, Sri Aurobindo Institute of Medical Sciences, Indore, Madhya Pradesh, India.
Aim:
To evaluate whether semen processing at 37°C yield sperm with better DNA integrity compared to centrifugation and processing at room temperature (RT) by swim-up method.
Settings:
This study was done at tertiary care center attached to Reproductive Medicine Unit and Medical College.
Design:
Prospective pilot study.
Patients:
Normozoospermic men (n = 50) undergoing diagnostic semen analysis.
Materials And Methods:
Normozoospermic samples (World Health Organization, 2010 criteria) after analysis was divided into two aliquots (0.5 mL each); one was processed at 37°C and the other at RT by swim-up method. DNA fragmentation of both samples post wash was calculated by acridine orange method.
Statistical Analysis Used:
The values of sperm DNA fragmentation were represented as mean and standard error (mean ± SEM) of the mean. Paired t-test was used for calculating the sperm DNA integrity difference between post wash at RT and 37°C.
Results:
Statistically significant difference was not observed in post wash sperm DNA fragmentation values at 37°C compared to RT.
Conclusion:
Our data represents that there was no significant difference in sperm DNA fragmentation values of samples processed at 37°C and at RT. Hence, sperm processing at 37°C does not yield sperm with better DNA integrity compared to centrifugation and processing at RT.
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