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Updated: May 9, 2026

Identification of Rare Bacterial Pathogens by 16S rRNA Gene Sequencing and MALDI-TOF MS
Published on: July 11, 2016
Beyond identification: emerging and future uses for MALDI-TOF mass spectrometry in the clinical microbiology
Mari L DeMarco1, Bradley A Ford
1Department of Pathology and Laboratory Medicine, University of British Columbia, St. Paul's Hospital, Vancouver, BC, V6Z 1Y6, Canada.
Abstract:
The routine use of matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS) has revolutionized microorganism identification in the clinical microbiology laboratory. Building from these now common microorganism identification strategies, this review explores future clinical applications of MALDI-TOF MS. This includes practical approaches for laboratorians interested in implementing direct identification processing methods for MALDI-TOF detection of microbes in bloodstream infection (BSI) and urinary tract infection (UTI), as well as, post-analytical approaches for classifying MALDI-TOF spectral data to detect characteristics other and species-level identification (e.g. strain-level classification, typing, and resistance mechanisms).
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