Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Three-Dimensional Microscopy in Microbiology01:28

Three-Dimensional Microscopy in Microbiology

Three-dimensional imaging techniques are essential in cell biology, allowing researchers to visualize intricate cellular structures with high resolution. Two prominent methods, Differential Interference Contrast Microscopy (DIC) and Confocal Scanning Laser Microscopy (CSLM), provide distinct advantages for imaging live and thick specimens, respectively.Differential Interference Contrast MicroscopyDIC microscopy enhances contrast in transparent, unstained samples by converting phase...
Phase Contrast and Differential Interference Contrast Microscopy01:26

Phase Contrast and Differential Interference Contrast Microscopy

Phase-Contrast Microscopes
In-phase-contrast microscopes, interference between light directly passing through a cell and light refracted by cellular components is used to create high-contrast, high-resolution images without staining. It is the oldest and simplest type of microscope that creates an image by altering the wavelengths of light rays passing through the specimen. Altered wavelength paths are created using an annular stop in the condenser. The annular stop produces a hollow cone of...
Total Internal Reflection Fluorescence Microscopy01:05

Total Internal Reflection Fluorescence Microscopy

Total internal reflection fluorescence microscopy or TIRF is an advanced microscopic technique used to visualize fluorophores in samples close to a solid surface with a higher refractive index, such as a glass coverslip. TIRF only allows fluorophores in proximity to the solid surface to be excited. When light from a medium with a lower refractive index (such as air) hits the glass coverslip at a critical angle, the light undergoes total internal reflection stead of passing through the glass.

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

Closed-Loop Deep Brain Stimulation With Reinforcement Learning and Neural Simulation.

IEEE transactions on neural systems and rehabilitation engineering : a publication of the IEEE Engineering in Medicine and Biology Society·2024
Same author

EUV scatterometer with a high-harmonic-generation EUV source.

Optics express·2016
Same author

Characterization of high density through silicon vias with spectral reflectometry.

Optics express·2011
Same author

Reflectometer-based metrology for high-aspect ratio via measurement.

Optics express·2010
Same author

Scatterometry-based metrology with feature region signatures matching.

Optics express·2009
Same author

Overlay measurement using angular scatterometer for the capability of integrated metrology.

Optics express·2009

Related Experiment Video

Updated: May 8, 2026

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy (iPALM)
11:57

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy (iPALM)

Published on: December 1, 2016

Infrared differential interference contrast microscopy for 3D interconnect overlay metrology.

Yi-sha Ku1, Deh-Ming Shyu, Yeou-Sung Lin

  • 1Center for Measurement Standards, ITRI, Bldg. 12, 321 Sec. 2, Kuang Fu Rd., Hsinchu, Taiwan. yku@itri.org.tw

Optics Express
|August 14, 2013
PubMed
Summary

Measuring wafer bonding overlay in 3D interconnects is challenging. Infrared microscopy with differential interference contrast (DIC) offers a solution, achieving better than 1.0 micron accuracy for overlay measurements.

More Related Videos

Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules
09:45

Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules

Published on: August 8, 2019

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions
13:43

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

Published on: June 24, 2013

Related Experiment Videos

Last Updated: May 8, 2026

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy (iPALM)
11:57

Three-dimensional Super Resolution Microscopy of F-actin Filaments by Interferometric PhotoActivated Localization Microscopy (iPALM)

Published on: December 1, 2016

Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules
09:45

Implementation of Interference Reflection Microscopy for Label-free, High-speed Imaging of Microtubules

Published on: August 8, 2019

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions
13:43

Correlative Microscopy for 3D Structural Analysis of Dynamic Interactions

Published on: June 24, 2013

Area of Science:

  • Materials Science
  • Metrology
  • Semiconductor Manufacturing

Background:

  • 3D interconnect metrology faces challenges with opaque silicon wafers.
  • Conventional optical microscopy is insufficient for through-wafer measurements.
  • Accurate measurement of wafer bonding overlay is critical for advanced packaging.

Purpose of the Study:

  • To demonstrate infrared microscopy with DIC for measuring wafer bonding overlay.
  • To overcome limitations of optical microscopy in 3D interconnect metrology.
  • To develop a reliable method for assessing overlay accuracy in bonded wafers.

Main Methods:

  • Utilized infrared microscopy enhanced by differential interference contrast (DIC).
  • Employed 2D symmetric overlay marks on front and back sides of thinned wafers.
  • Developed a custom analysis algorithm and theoretical fitting model for overlay error mapping.

Main Results:

  • Successfully measured wafer bonding overlay using the developed infrared DIC method.
  • Quantified overlay error between bonded wafers and interconnect structures.
  • Achieved a measurement accuracy better than 1.0 micron.

Conclusions:

  • Infrared microscopy with DIC is effective for 3D interconnect metrology.
  • The developed method provides high accuracy for wafer bonding overlay assessment.
  • This technique addresses a key challenge in semiconductor manufacturing metrology.