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Updated: May 7, 2026

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Using the E1A Minigene Tool to Study mRNA Splicing Changes
Published on: April 22, 2021
Splicing and alternative splicing in rice and humans
Zhiguo E1, Lei Wang, Jianhua Zhou
1Nantong University, Nantong 226001, P.R. China jianhua@ntu.edu.cn; jianhua55@msn.com.
BMB Reports
|September 26, 2013
Summary
Rice gene alternative splicing is crucial for adapting to climate change-induced abiotic stresses. Understanding splicing mechanisms in rice offers insights for improving crop resilience and functional genomics.
Area of Science:
- Genomics
- Plant Biology
- Molecular Biology
Background:
- Rice is a vital monocot gramineous crop and a model for other cereals.
- Climate change and soil degradation cause significant abiotic stresses impacting agriculture.
- Alternative splicing in rice is increasingly recognized as a response to environmental stress.
Purpose of the Study:
- To review recent advancements in rice splicing and alternative splicing research.
- To compare rice splicing mechanisms with those in humans.
- To explore the implications of splicing research for rice functional genomics and crop improvement.
Main Methods:
- Review of existing literature on rice splicing.
- Analysis of data from microarrays and next-generation sequencing.
- Comparative analysis of splicing in rice and humans.
Main Results:
- Over half of rice genes undergo alternative splicing.
- Alternative splicing patterns are significantly affected by abiotic stress conditions.
- Splicing research provides a pathway for enhancing rice's adaptation to stress.
Conclusions:
- Manipulating alternative splicing is a promising strategy for developing stress-resilient rice.
- Further research on rice splicing will advance functional genomics and genetic improvement.
- Understanding alternative splicing is key to addressing agricultural challenges posed by climate change.
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Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
Alternative RNA Splicing
Alternative RNA splicing is the regulated splicing of exons and introns to produce different mature mRNAs from a single pre-mRNA. Unlike in constitutive splicing where a single gene produces a single type of mRNA, alternative splicing allows an organism to produce multiple proteins from a single gene and plays an important role in protein diversity.
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
There are five types of alternative RNA splicing that vary in the ways the pre-mRNA segments are removed or retained in the mature mRNA. The first...
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
RNA Splicing
Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
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Splicing is the process by which eukaryotic RNA is edited before its translation into protein. The RNA strand transcribed from eukaryotic DNA is called the primary transcript. The primary transcripts that become mRNAs are called precursor messenger RNAs (pre-mRNAs). Eukaryotic pre-mRNA contains alternating sequences of exons and introns. Exons are nucleotide sequences that code for proteins, whereas introns are the non-coding regions. In RNA splicing, introns are removed and exons are bonded...
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In eukaryotic cells, transcripts made by RNA polymerase are modified and processed before exiting the nucleus. Unprocessed RNA is called precursor mRNA or pre-mRNA to distinguish it from mature mRNA.
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
Once about 20-40 ribonucleotides have been joined together by RNA polymerase, a group of enzymes adds a “cap” to the 5’ end of the growing transcript. In this process, a 5’ phosphate is replaced by modified guanosine that has a methyl group attached to it (7-Methyl guanosine). This 5’ cap helps the...
