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In Vitro Culture Strategy for Oocytes from Early Antral Follicle in Cattle
Published on: July 8, 2020
Primate follicular development and oocyte maturation in vitro
Jing Xu1, Min Xu, Marcelo P Bernuci
1Division of Reproductive and Developmental Sciences, Oregon National Primate Research Center, Oregon Health & Science University, 505 NW 185th Avenue, Beaverton, OR, 97006, USA, xujin@ohsu.edu.
Advances in Experimental Medicine and Biology
|October 8, 2013
Summary
This review explores in vitro follicle maturation (IFM) in primates using 3D culture systems. It highlights advances in studying folliculogenesis and preserving fertility in cancer patients.
Area of Science:
- Reproductive Biology
- Developmental Biology
- Cell Biology
Background:
- Primate follicular development is complex and not fully understood.
- In vitro follicle maturation (IFM) offers fertility preservation options for cancer patients.
- Three-dimensional (3D) follicle culture systems provide a model for studying primate folliculogenesis.
Purpose of the Study:
- To review published data on in vitro follicular development in nonhuman primates.
- To explore the potential of 3D culture systems for studying primate folliculogenesis.
- To identify strategies for improving in vitro follicle maturation for fertility preservation.
Main Methods:
- Review of published literature on in vitro follicular development in nonhuman primates.
- Analysis of data on follicle survival, growth, and function in 3D culture.
- Evaluation of oocyte maturation and fertilization rates in vitro.
Main Results:
- Summarizes current knowledge on preantral to small antral stage follicular development in vitro.
- Discusses endocrine and paracrine/autocrine regulation of folliculogenesis.
- Highlights challenges and strategies for improving oocyte competence for fertilization and embryonic development.
Conclusions:
- 3D follicle culture systems are valuable for studying primate folliculogenesis.
- IFM holds promise for fertility preservation in female cancer patients.
- Further research is needed to optimize conditions for human follicle culture and clinical application.
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