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Updated: May 6, 2026

Microbiota Analysis Using Two-step PCR and Next-generation 16S rRNA Gene Sequencing
Published on: October 15, 2019
Mark Pimentel1, Ruchi Mathur, Christopher Chang
1GI Motility Program, Division of Gastroenterology, Cedars-Sinai Medical Center, 8730 Alden Drive, Suite 201E, Los Angeles, CA, 90048, USA, pimentelm@cshs.org.
Human gut microbes produce gases like hydrogen (H2), methane (CH4), and hydrogen sulfide (H2S). These gases impact gut health and can serve as diagnostic markers for conditions such as bacterial overgrowth.
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