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Direct localization and quantitation of aminoacyl-tRNA synthetase activity in polyacrylamide gel
Analytical Biochemistry
|September 1, 1985
Abstract:
A simple procedure for the direct localization and quantitation of aminoacyl-tRNA synthetase activity in polyacrylamide gel is described. The method is based on the white precipitation of Ca-PPi. The white bands of the precipitated calcium salt are clearly visible when viewed against a dark background and can be photographed or scanned.