Loose patch clamp recording of ionic currents in demyelinated frog nerve fibers

Ionic currents in demyelinated segments of frog sciatic nerves have been detected by means of a patch clamp technique. Axons were examined 6-13 days after lysolecithin-induced demyelination. Both voltage-gated sodium channels and voltage-gated potassium channels could be demonstrated in the demyelinated axolemma.