A tetracycline-inducible expression vector for Streptococcus agalactiae allowing controllable gene expression
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Updated: May 6, 2026

Applying an Inducible Expression System to Study Interference of Bacterial Virulence Factors with Intracellular Signaling
Published on: June 25, 2015
Marie-Frédérique Lartigue1, Philippe Bouloc2
1Institut de Génétique et Microbiologie, CNRS/UMR 8621, Centre scientifique d'Orsay, Université Paris-Sud, Bâtiment 400, 91405 Orsay Cedex, France; CHRU de Tours, Université François Rabelais de Tours, UMR1282 ISP, F-37032 Tours, France; INRA, UMR1282 ISP, F-37380 Nouzilly, France.
Abstract:
Streptococcus agalactiae is the principal cause of neonatal infections; however, for its study, genetic tools are scarce. We have developed pG+off, an expression vector with a tetracycline inducible promoter that allows a tightly-controllable gene expression in S. agalactiae. Using a reporter gene, we have shown that pG+off is characterized by tight repression under non-induced condition.
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