Development and validation of method for heterocyclic compounds in wine: optimization of HS-SPME conditions applying
Vívian Maria Burin1, Stéphanie Marchand, Gilles de Revel
1Université de Bordeaux, ISVV, EA 4577, Unité de Recherche Oenologie, 33882 Villenave d'Ornon, France; INRA, ISVV, USC 3666 oenologie, 33882 Villenave d'Ornon, France; Departamento de Ciência e Tecnologia de Alimentos CAL/CCA, Universidade Federal de Santa Catarina, Rod. Admar Gonzaga, 1346, Itacorubi, CEP 88034-001 Florianópolis, SC, Brazil.
Abstract:
Considering the importance of the heterocyclic compounds in terms of wine flavor, this study aims to propose a new rapid and solvent free method to quantify different classes of heterocyclic compounds, such as furans, thiophenes, thiazols and pyrazines, which are products of the Maillard reaction, in wines. The use of a central composite design and the response surface methodology to determine the best conditions allows the optimum combination of analytical variables (pH, NaCl and extraction time) to be identified. The validation was carried out using several types of wine as matrices. The method shows satisfactory repeatability (2.7%
More Related Videos
Related Concept Videos
Methods of Medium Optimization
Optimizing Chromatographic Separations
Band broadening refers to spreading solute bands as they travel through the column. This broadening can impact resolution. Plate height (H) represents the length required for one theoretical plate. A lower plate height corresponds to...


