Jove
Visualize
Contact Us
JoVE
x logofacebook logolinkedin logoyoutube logo
ABOUT JoVE
OverviewLeadershipBlogJoVE Help Center
AUTHORS
Publishing ProcessEditorial BoardScope & PoliciesPeer ReviewFAQSubmit
LIBRARIANS
TestimonialsSubscriptionsAccessResourcesLibrary Advisory BoardFAQ
RESEARCH
JoVE JournalMethods CollectionsJoVE Encyclopedia of ExperimentsArchive
EDUCATION
JoVE CoreJoVE BusinessJoVE Science EducationJoVE Lab ManualFaculty Resource CenterFaculty Site
Terms & Conditions of Use
Privacy Policy
Policies

Related Concept Videos

Regulation of Hematopoietic Stem Cells01:01

Regulation of Hematopoietic Stem Cells

3.5K
All blood and immune cells are produced from the multipotent hematopoietic stem cells (HSCs) by the process of hematopoiesis. However, they all have a limited life span. In addition, many are depleted in immune surveillance or combatting an injury or infection. This makes blood one of the most regenerative tissues. Hematopoiesis helps replenish these blood and immune cells, restoring the body's normal functioning. However, overproduction of blood and immune cells can make them cancerous or...
3.5K
Multipotency of Hematopoietic Stem Cells01:19

Multipotency of Hematopoietic Stem Cells

3.1K
The hematopoietic stem cells or HSCs are multipotent, meaning they can differentiate and give rise to all blood and immune cells. HSCs are maintained in the quiescent stage until an external stimulus initiates their differentiation. The multipotent HSCs exist as two heterogeneous populations, long-term repopulating cells (LTRC) and short-term repopulating cells (STRC). The two HSC populations have different surface markers or receptors and are classified based on quiescence and long-term...
3.1K
Lineage Commitment01:21

Lineage Commitment

3.4K
Commitment is the  process whereby stem cells:
3.4K
Overview of Hematopoiesis01:20

Overview of Hematopoiesis

9.9K
Hematopoiesis, or blood cell production, is a vital biological process that begins early in embryonic development and continues throughout life. This process generates the various types of cells found in blood, including red blood cells, white blood cells, and platelets from hematopoietic stem cells (HSCs).
Developmental Phases of Hematopoiesis
Initially, HSCs are formed in the embryonic yolk sac, a critical site for early blood cell production. These stem cells subsequently migrate to other...
9.9K
Combinatorial Gene Control02:33

Combinatorial Gene Control

8.6K
Combinatorial gene control is the synergistic action of several transcriptional factors to regulate the expression of a single gene. The absence of one or more of these factors may lead to a significant difference in the level of gene expression or repression.
The expression of more than 30,000 genes is controlled by approximately 2000-3000 transcription factors. This is possible because a single transcription factor can recognize more than one regulatory sequence. The specificity in gene...
8.6K
Hematopoiesis01:21

Hematopoiesis

8.1K
The process of blood cell formation is called hematopoiesis. Hematopoiesis starts early during development, on the seventh day of embryogenesis. This phase of hematopoiesis is called the primitive wave, wherein the extraembryonic yolk sac allows the production of erythroid cells and endothelial cells from a common precursor called hemangioblast. The erythroid cells provide oxygen to support the growth of the rapidly dividing embryo. Hemangioblasts later develop into hematopoietic stem cells or...
8.1K

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by
Same author

The link between father presence and critical thinking disposition of college students: The mediating role of self-esteem and the moderating role of gender.

Acta psychologica·2026
Same author

Radiolabeled Coordination Polymer-Loaded Microneedles for Synergistic Melanoma Brachytherapy-Immunotherapy via STING Activation and Pyroptosis.

Exploration (Beijing, China)·2026
Same author

Nitrate contamination in a karst aquifer affected by acid mine drainage during a storm event: Insights from stable isotopes and hydrochemical tracers.

Journal of hazardous materials·2026
Same author

Predicting heterosexual reactions to coming out in China.

BMC psychology·2026
Same author

Corrigendum to "RJT-101, a novel camptothecin derivative, is highly effective in the treatment of melanoma through DNA damage by targeting topoisomerase 1" [Biochem. Pharmacol. 171 (2020) 113716].

Biochemical pharmacology·2026
Same author

Development and Evaluation of a Promising Biomarker for Diagnosis of Latent and Active Tuberculosis Infection.

Infectious diseases & immunity·2026
Same journal

Psychological stress drives aging-like hematopoietic stem cell dysfunction through a brain-gut-bone marrow axis.

Cell stem cell·2026
Same journal

Human stem cell-based embryo model governance: Insights from Japan.

Cell stem cell·2026
Same journal

From reconstruction to intervention: Engineered organoids as living therapeutic depots.

Cell stem cell·2026
Same journal

Long-term lessons from MATCH01 macrophage therapy in cirrhosis.

Cell stem cell·2026
Same journal

Beyond apoptosis: LSC state dictates metabolic and anti-apoptotic vulnerabilities.

Cell stem cell·2026
Same journal

Outside the niche: Gut microbiota relay psychological stress to hematopoietic stem cell dysfunction.

Cell stem cell·2026
See all related articles

Related Experiment Video

Updated: May 5, 2026

Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation
11:40

Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation

Published on: October 20, 2014

8.1K

Polycomb repressive complex 2 regulates normal hematopoietic stem cell function in a developmental-stage-specific

Huafeng Xie1, Jian Xu1, Jessie H Hsu1

  • 1Department of Pediatric Oncology, Dana-Farber Cancer Institute and Division of Hematology/Oncology, Boston Children's Hospital, Harvard Stem Cell Institute, Harvard Medical School, Boston, MA 02115, USA.

Cell Stem Cell
|November 19, 2013
PubMed
Summary
This summary is machine-generated.

Deleting Eed, a Polycomb repressive complex 2 (PRC2) subunit, exhausts adult hematopoietic stem cells (HSCs) but not fetal ones. This reveals stage-specific PRC2 requirements for HSC function.

More Related Videos

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells
10:21

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Published on: February 21, 2018

9.3K
Clonal Analysis of Embryonic Hematopoietic Stem Cell Precursors Using Single Cell Index Sorting Combined with Endothelial Cell Niche Co-culture
09:32

Clonal Analysis of Embryonic Hematopoietic Stem Cell Precursors Using Single Cell Index Sorting Combined with Endothelial Cell Niche Co-culture

Published on: May 8, 2018

8.0K

Related Experiment Videos

Last Updated: May 5, 2026

Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation
11:40

Retroviral Infection of Murine Embryonic Stem Cell Derived Embryoid Body Cells for Analysis of Hematopoietic Differentiation

Published on: October 20, 2014

8.1K
Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells
10:21

Proliferation and Differentiation of Murine Myeloid Precursor 32D/G-CSF-R Cells

Published on: February 21, 2018

9.3K
Clonal Analysis of Embryonic Hematopoietic Stem Cell Precursors Using Single Cell Index Sorting Combined with Endothelial Cell Niche Co-culture
09:32

Clonal Analysis of Embryonic Hematopoietic Stem Cell Precursors Using Single Cell Index Sorting Combined with Endothelial Cell Niche Co-culture

Published on: May 8, 2018

8.0K

Area of Science:

  • Developmental biology
  • Stem cell biology
  • Epigenetics

Background:

  • Polycomb repressive complex 2 (PRC2) is crucial for stem cell maintenance and cancer.
  • Previous studies using loss-of-function for individual PRC2 subunits yielded conflicting results.
  • Eed is a core subunit essential for the assembly of PRC2 complexes containing both Ezh1 and Ezh2.

Purpose of the Study:

  • To investigate the role of PRC2 in hematopoiesis by conditionally deleting the core subunit Eed.
  • To determine the developmental stage-specific functions of PRC2 in hematopoietic stem cells (HSCs).

Main Methods:

  • Conditional deletion of the Eed gene in mice.
  • Assessment of hematopoiesis in fetal liver and adult bone marrow.
  • Analysis of hematopoietic stem cell (HSC) function, including maintenance and differentiation.
  • Comparative gene expression profiling of Eed-null HSCs.
  • Genetic deletion of Cdkn2a in Eed-null mice.

Main Results:

  • Conditional deletion of Eed leads to exhaustion of adult bone marrow HSCs.
  • Fetal liver HSCs are produced in normal numbers despite Eed deletion.
  • Neonatal Eed-null HSCs express HSC signature genes but exhibit defects in maintenance and differentiation.
  • Gene expression profiling reveals upregulation of conflicting pathways in Eed-null HSCs.
  • Deletion of Cdkn2a improves HSPC survival but does not restore HSC function in Eed-null mice.

Conclusions:

  • Canonical PRC2 complexes have distinct, developmental stage-specific requirements for normal HSC function.
  • Eed is essential for maintaining adult HSC function, but not for fetal HSC development.
  • PRC2 regulates complex gene expression programs that are critical for HSC homeostasis.