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Updated: May 5, 2026

Induction and Testing of Hypoxia in Cell Culture
Published on: August 12, 2011
Hypoxia and regulation of cancer cell stemness
1Department of Therapeutic Radiology, Yale School of Medicine, 208040, New Haven, 06520-8040, CT, USA, zhong.yun@yale.edu.
Abstract:
Spontaneous tumors often contain heterogeneous populations of tumor cells with different tumor-initiating potentials or cancer cell "stemness." Clonal heterogeneity can be traced to specific locations inside a tumor where clones with different metastatic capabilities are identified, suggesting that the tumor microenvironment can exert a significant effect on the evolution of different clonal populations. Hypoxia is a common feature of tumor microenvironments and has the potential to facilitate malignant progression. This chapter provides a synopsis of hypoxia-regulated pathways implicated in the maintenance of cancer stem cells.
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