Search research articles

ABOUT JoVE

Overview Leadership Blog JoVE Help Center

AUTHORS

Publishing Process Editorial Board Scope & Policies Peer Review FAQ Submit

LIBRARIANS

Testimonials Subscriptions Access Resources Library Advisory Board FAQ

RESEARCH

JoVE Journal Methods Collections JoVE Encyclopedia of Experiments Archive

EDUCATION

JoVE Core JoVE Business JoVE Science Education JoVE Lab Manual Faculty Resource Center Faculty Site

Terms & Conditions of Use

Related Concept Videos

Vesicular Tubular Clusters

Vesicular Tubular Clusters

After budding out from the ER membrane, some COPII vesicles lose their coat and fuse with one another to form larger vesicles and interconnected tubules called vesicular tubular clusters or VTCs. These clusters constitute a compartment at the ER-Golgi interface known as ERGIC (Endoplasmic Reticulum Golgi Intermediate Compartment). The ERGIC is a mobile membrane-bound cargo transport system that sorts proteins secreted from ER and delivers them to the Golgi.
With the help of motor proteins such...

You might also read

Related Articles

Articles linked to this work by shared authors, journal, and citation graph.

Sort by

Same author

Alkalization and cell wall remodeling mark the fruit abscission zone maturation in table olive.

Journal of experimental botany·2026

Same author

systemPipeR: a multipurpose workflow management system for reproducible data analysis.

NAR genomics and bioinformatics·2026

Same author

Unusually broad-spectrum small-molecule sensing using a single protein scaffold.

Proceedings of the National Academy of Sciences of the United States of America·2025

Same author

Cell layer-specific cell wall modification is associated with exo-mesocarp split in pistachio (Pistacia vera L.).

Journal of experimental botany·2025

Same author

Best practices in plant fluorescence imaging and reporting: A primer.

The Plant cell·2025

Same author

Natural variation in salt-induced changes in root:shoot ratio reveals SR3G as a negative regulator of root suberization and salt resilience in <i>Arabidopsis</i>.

eLife·2025

Same journal

Isolation of Mesenchymal Stem Cell-Derived Extracellular Vesicles.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Modeling Melanoma Immune Surveillance by CAR-T Cells in Human Skin Organoids.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Stepwise Optimization of a Matrigel-Based In Vitro Angiogenesis Assay for Reproducible and Quantifiable 2D-Tube Formation Using HUVECs.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Quantifying Mechanical Properties of Fresh Ovarian Tissue with Optical Brillouin Microscopy.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

3D Chromatin Architecture During Early Development: New Methods and New Findings.

Methods in molecular biology (Clifton, N.J.)·2026

Same journal

Metabolic Plasticity in Embryogenesis Throughout the Lens of NAD<sup></sup>.

Methods in molecular biology (Clifton, N.J.)·2026

See all related articles

Search research articles

Related Experiment Video

Updated: May 5, 2026

Formation of Biomembrane Microarrays with a Squeegee-based Assembly Method

Formation of Biomembrane Microarrays with a Squeegee-based Assembly Method

Published on: May 8, 2014

Endomembrane dissection using chemically induced bioactive clusters.

Natasha Worden¹, Thomas Girke, Georgia Drakakaki

¹Department of Plant Sciences, University of California, Davis, CA, USA.

Methods in Molecular Biology (Clifton, N.J.)

|December 6, 2013

Summary

This summary is machine-generated.

This study introduces a chemical genomics method for plant research, enabling the discovery of new chemicals that affect cell functions and aiding in pathway analysis.

More Related Videos

Cryosectioning Method for Microdissection of Murine Colonic Mucosa

Cryosectioning Method for Microdissection of Murine Colonic Mucosa

Published on: July 12, 2015

Perturbing Endothelial Biomechanics via Connexin 43 Structural Disruption

Perturbing Endothelial Biomechanics via Connexin 43 Structural Disruption

Published on: October 4, 2019

Related Experiment Videos

Last Updated: May 5, 2026

Formation of Biomembrane Microarrays with a Squeegee-based Assembly Method

Formation of Biomembrane Microarrays with a Squeegee-based Assembly Method

Published on: May 8, 2014

Cryosectioning Method for Microdissection of Murine Colonic Mucosa

Cryosectioning Method for Microdissection of Murine Colonic Mucosa

Published on: July 12, 2015

Perturbing Endothelial Biomechanics via Connexin 43 Structural Disruption

Perturbing Endothelial Biomechanics via Connexin 43 Structural Disruption

Published on: October 4, 2019

Area of Science:

Plant biology
Chemical genomics
Cell biology

Background:

Chemical genomics offers rapid functional analysis of plant components.
Previous chemical screens for subcellular phenotypes were limited in plants.

Purpose of the Study:

To describe a systematic chemical screen for plant plasma membrane recycling markers.
To identify novel chemicals for plant pathway dissection and endomembrane system research.

Main Methods:

Systematic chemical screen of plasma membrane recycling markers in plants.
Utilizing confocal microscopy to observe subcellular phenotypes.
Clustering of observed subcellular phenotypes to identify chemical effects.

Main Results:

Successfully adapted chemical screening for plant subcellular phenotypes.
Identified chemicals with desired effects on plasma membrane recycling.
Established a method for discovering novel chemical probes for endomembrane trafficking.

Conclusions:

This protocol enhances the accessibility of chemical genomics in plant science.
The developed method aids in dissecting plant pathways and understanding the endomembrane system.
Accumulation of compartment-specific markers and chemical probes advances endomembrane trafficking research.