Combined surface plasmon resonance and impedance spectroscopy systems for biosensing

Area of Science:

• Biosensing
• Nanotechnology
• Spectroscopy

Background:

• Interdigitated electrodes (IDEs) are crucial components in advanced biosensing platforms.
• Surface plasmon resonance (SPR) and electrochemical impedance spectroscopy (EIS) are powerful analytical techniques.
• Integrating optical and electrochemical methods offers synergistic advantages for biosensing.

Purpose of the Study:

• To develop a novel combined surface plasmon resonance and electrochemical impedance spectroscopy (SP-EIS) biosensing system.
• To utilize interdigitated metal electrodes (IDEs) as both SPR supporting structures and optical gratings.
• To demonstrate a simplified optical setup for direct surface plasmon spectral analysis.

Main Methods:

• Fabrication of sensor chips with micrometer-sized interdigitated metal electrodes (IDEs).
• Integration of SPR and EIS techniques on a single platform.
• Utilizing the diffraction properties of IDEs for spectral analysis of surface plasmons.

Main Results:

• Successful development of combined SP-EIS biosensing systems.
• Demonstration of IDEs functioning as SPR supporting structures and diffraction gratings.
• Achieved direct surface plasmon spectral analysis via first-order diffraction, enabling simplified optical setups.

Conclusions:

• SP-EIS biosensing represents a promising technology for conductometric and SPR sensing.
• The developed system is suitable for both portable and stationary sensing applications.
• This approach offers a novel and simplified method for SPR spectral analysis in biosensing.