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An oligofluorene truxene based distributed feedback laser for biosensing applications.
Anne-Marie Haughey1, Benoit Guilhabert1, Alexander L Kanibolotsky2
1Institute of Photonics, SUPA, University of Strathclyde, Glasgow G4 0NW, UK.
Biosensors & Bioelectronics
|December 21, 2013
Summary
A novel organic laser biosensor detects specific protein-small molecule interactions, like avidin, with high sensitivity and reversibility. This platform offers a reusable and modular approach for biomolecule detection.
Area of Science:
- Organic electronics
- Biomolecular sensing
- Laser technology
Background:
- Distributed feedback (DFB) lasers are crucial for sensing applications.
- Organic materials offer tunable properties for novel device fabrication.
- Protein-small molecule interactions are key targets in diagnostics.
Purpose of the Study:
- To report the first all-organic oligofluorene truxene based DFB laser for detecting protein-small molecule interactions.
- To develop a sensitive and selective biosensor platform for avidin detection.
Main Methods:
- Fabrication of an all-organic DFB laser using oligofluorene truxene.
- Immobilization of biotin on the laser surface to create a biosensor.
- Detection of avidin binding through changes in laser output.
- Testing selectivity and reversibility using desthiobiotin and Bovine Serum Albumin (BSA).
Main Results:
- The biosensor successfully detected avidin down to 1 μg mL(-1).
- The interaction was selective and reversible, confirmed by using desthiobiotin.
- Avidin detection was unaffected by high concentrations of BSA (up to 50,000 μg mL(-1)).
Conclusions:
- An all-organic DFB laser biosensor platform has been developed.
- The platform demonstrates high sensitivity, selectivity, and reversibility for avidin detection.
- This offers a promising, modular, and potentially reusable approach for biomolecule sensing.
