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Updated: May 4, 2026

Cell-free Biochemical Fluorometric Enzymatic Assay for High-throughput Measurement of Lipid Peroxidation in High Density Lipoprotein
Published on: October 12, 2017
Oxidative modification of lipoproteins.
1Department of Biotechnology and Environmental Chemistry, Kitami Institute of Technology, Kitami, Hokkaido, 090-8507, Japan, araihrfm@mail.kitami-it.ac.jp.
Oxidation of lipoproteins, particularly low-density lipoprotein (LDL), by reactive oxygen species (ROS) is linked to disease. This study details oxidative modifications of apolipoprotein B100 (apo B100) in LDL.
Area of Science:
- Biochemistry
- Molecular Biology
- Cardiovascular Research
Background:
- Lipoproteins, composed of lipids and apolipoproteins, are crucial for lipid metabolism.
- Oxidation of lipoproteins by reactive oxygen species (ROS) is implicated in various diseases.
- Low-density lipoprotein (LDL) oxidation and its link to atherosclerosis are extensively studied.
Purpose of the Study:
- To describe the characteristics and oxidation mechanisms of lipoproteins by ROS.
- To focus on oxidative modifications of apolipoprotein B100 (apo B100) in LDL.
- To report on oxidative modifications of apo B100 by lipid hydroperoxides.
Main Methods:
- In vitro and in vivo studies of lipoprotein oxidation.
- Characterization of oxidative modifications of apo B100 in LDL.
- Immunological techniques to confirm apo B100 modifications in oxidized LDL and atherosclerotic lesions.
Main Results:
- Polyunsaturated fatty acid residues in lipids and apolipoproteins are primary targets of lipoprotein oxidation.
- Oxidative modifications of apo B100 have been extensively characterized.
- Modifications of apo B100 by oxidized lipids are confirmed in oxidized LDL and atherosclerotic lesions.
Conclusions:
- Lipoprotein oxidation by ROS plays a role in disease pathogenesis.
- Apo B100 is a key target for oxidative modification within LDL.
- Lipid hydroperoxides are significant in the early stages of apo B100 oxidative modification.
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