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[Effect of Solanum nigrum on human colon carcinoma RKO cells ]
Bing Hu1, Hong-Mei An2, Ke-Ping Shen2
1Longhua Hospital, Shanghai University of Traditional Chinese Medicine, Shanghai 200032, China. beearhu@hotmail.com
Objective:
To evaluate the effect of Solanum nigrum on adhesion, migration and invasion in human colon carcinoma RKO cells.
Methods:
RKO cells were treated with different dose of Solanum nigrum. Cell proliferation was detected by CCK-8 assay. Cell adhesion was observed with CytoSelect 48-Well Cell Adhesion Assay. Cell migration was detected with scratch assay. Cell invasion was analyzed by CytoSelect 24-Well Cell Invasion Assay.
Results:
At final concentration of 400-1600 microg/mL, Solanum nigrum significantly inhibited proliferation of RKO cells in a dose-dependent manner. At final concentration of 100-400 microg/mL, Solanum nigrum significantly inhibited adhesion,migration and invasion in RKO cells.
Conclusion:
Solanum nigrum may inhibit the proliferation, adhesion, migration and invasive abilities in RKO cells. The present study provides new insight into the application of Solanum nigrum for colon carcinoma treatment that are worthy of further study.
