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Updated: May 4, 2026
Dried Blood Spots - Preparing and Processing for Use in Immunoassays and in Molecular Techniques
Published on: March 13, 2015
Ammonium improves elution of fixed dried blood spots without affecting immunofluorescence assay quality
1Evolutionary Ecology Group, University of Antwerp, Antwerp, Belgium.
Objective:
To solve the problem of fixed dried blood spot elution without damaging IgG antibodies.
Methods:
The minimum effective concentration of liquid ammonium (NH3 ) in a PBS solution, which was found to elute fixed blood, was determined. By using a dilution series, the effects of NH3 on IgG antibody quality were assessed using immunofluorescence assays.
Results:
The minimum effective concentration of 0.2% NH3 has no detectable effects on IgG quality.
Conclusion:
Ammonium greatly improves blood elution from fixed DBS while maintaining IgG antibody quality. These results are encouraging and provide a basis for further testing of the efficacy of ammonium in different situations as well as its effect on other compounds.
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