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Updated: May 4, 2026
Isolation of Primary Human Decidual Cells from the Fetal Membranes of Term Placentae
Published on: April 30, 2018
[Isolation, culture and identification of sheep multinucleated chorionic trophoblast cells]
Zhuanjia Wang1, Yufei Zhang1, Shuying Liu2
1College of Veterinary Medicine, Inner Mongolia Agricultural University, Ministry of Agriculture, Huhhot 010018, China.
Objective:
To develop a set of methods for isolating and culturing sheep multinucleated chorionic trophoblast cells and identify them.
Methods:
Trophoblast cells were isolated and cultured by two-step digestion of trypsin and collagenase, and observed under an inverted phase-contrast microscope. They were further identified through conventional HE staining, immunohistochemical staining and transmission electron microscopy.
Results:
Under an inverted phase-contrast microscope, the obtained trophoblast cells exhibited epithelioid and sheet-like spreading growth with binuclear or multinuclear. The multinucleated trophoblast cytoplasm from the sheep placental cotyledons and the slides of the trophoblast cells were all stained brown and exhibited positive reactivity by immunohistochemical staining with cytokeratin antibodies. Also abundance of microvilli on surface of cells, together with intracytoplasmic vacuoles, microfilament and lipid droplets were observed under a transmission electron microscope.
Conclusion:
Two-step digestion of trypsin and collagenase has been established for the isolation and cultivation of sheep multinucleated trophoblast cells, and using it, we obtained sheep multinucleated chorionic trophoblast cells with a high purity and biological activity.
