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Surface Enhanced Raman Spectroscopy Detection of Biomolecules Using EBL Fabricated Nanostructured Substrates

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Published on: March 20, 2015

Reusable three-dimensional nanostructured substrates for surface-enhanced Raman scattering.

Zhendong Zhu, Qunqing Li¹, Benfeng Bai

¹Department of Physics & Tsinghua-Foxconn Nanotechnology Research Center, State Key Laboratory of Low-Dimensional Quantum Physics, Tsinghua University, Beijing 100084, China. QunqLi@mail.tsinghua.edu.cn.

Nanoscale Research Letters

|January 15, 2014

Summary

This summary is machine-generated.

Researchers developed a simple, low-cost method using nanosphere lithography (NSL) to create 3D nanostructured substrates. These substrates achieve ultra-high surface-enhanced Raman scattering (SERS) sensitivity, enabling single-molecule detection.

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Area of Science:

Materials Science
Nanotechnology
Spectroscopy

Background:

Fabricating 3D nanostructured substrates with small nanogaps is challenging with conventional methods.
Existing techniques often involve laborious and complex procedures.
Adhesion layers like Chromium (Cr) or Titanium (Ti) can negatively impact performance.

Purpose of the Study:

To develop a simple, low-cost, large-area, and spatially controllable method for fabricating 3D nanostructures.
To utilize these nanostructures as substrates for ultra-sensitive surface-enhanced Raman scattering (SERS).
To overcome limitations associated with intermediate adhesion layers in SERS substrates.

Main Methods:

Employed nanosphere lithography (NSL) for fabricating various 3D nanostructures (hemisphere, hemiellipsoid, pyramidal pits).
Utilized Rhodamine 6G (R6G) as a single-molecule analyte.
Investigated the SERS performance of the fabricated nanostructured quartz substrates with different metal films.

Main Results:

Achieved an average SERS enhancement factor of up to 10^11.
Successfully detected single Rhodamine 6G molecules.
Demonstrated that the 3D nanostructure design mitigates the negative effects of adhesion layers.

Conclusions:

The developed NSL-based method offers a facile and scalable approach to create advanced 3D nanostructured SERS substrates.
The nanostructured quartz platform is versatile, allowing modification with various metal films and is recyclable.
This technique provides a cost-effective and efficient alternative to traditional fabrication methods for high-performance SERS applications.