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Updated: May 3, 2026

Optimized Quantitative Assessment of Enhancer RNA Stability in Mouse Embryonic Stem Cells
Published on: November 21, 2025
Methyltransferases modulate RNA stability in embryonic stem cells
Shuibin Lin1, Richard I Gregory1
1Stem Cell Program, Boston Children's Hospital, Massachusetts 02115, USA; the Department of Biological Chemistry and Molecular Pharmacology, and Department of Pediatrics, Harvard Medical School, Boston, Massachusetts 02115, USA; and the Harvard Stem Cell Institute, Boston, Massachusetts 02115, USA.
Abstract:
Emerging data support that RNA methylation plays important roles in RNA processing and metabolism. The methyltransferases Mettl3 and Mettl14 are shown to catalyse N(6)-methyladenosine (m(6)A) RNA modification in embryonic stem cells (ESCs). This m(6)A modification controls RNA metabolism and functions to destabilize mRNAs encoding developmental regulators to help sustain ESC self-renewal.
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