The intersection of flow cytometry with microfluidics and microfabrication

Area of Science:

• Analytical Chemistry
• Biotechnology
• Microfluidics

Background:

• Flow cytometry enables high-throughput analysis of particles, measuring optical and non-optical parameters.
• Advanced flow cytometers offer capabilities like single molecule detection and spectral imaging.
• Microfluidics has significantly impacted bio-analytical tools, including flow cytometry.

Purpose of the Study:

• To detail the functions and applications of modern flow cytometers.
• To review the historical and recent contributions of microfluidics to flow cytometry.
• To explore opportunities for integrating microfabrication with flow cytometry.

Main Methods:

• Analysis of particle characteristics using optical and non-optical measurements.
• High-speed particle sorting and analysis at rates up to 50,000 particles per second.
• Integration of microfluidic devices for enhanced flow cytometry performance.

Main Results:

• Flow cytometers can measure multiple fluorescence channels and scattered light.
• Specialized systems provide advanced analysis like single molecule detection and spectral collection.
• Applications span immunophenotyping, cell counting, screening, and rare cell analysis.

Conclusions:

• Flow cytometry is a versatile tool with broad applications in life sciences.
• Microfluidics and microfabrication offer significant potential for advancing flow cytometry.
• Synergistic integration promises further innovation in high-performance bio-analysis.