Validation of spectrophotometric method for Se(IV) determination: analytical applications

Area of Science:

• Analytical Chemistry
• Environmental Science
• Biochemistry

Background:

• Selenium is crucial for antioxidant enzyme function.
• Selenium deficiency is potentially linked to cancer.
• Accurate Se(IV) quantification is essential for health and environmental monitoring.

Purpose of the Study:

• To develop and validate a spectrophotometric method for Se(IV) assay.
• To establish optimal working conditions for the developed method.
• To apply the method for Se(IV) determination in various real-world samples.

Main Methods:

• Spectrophotometric determination of Se(IV) using N,N-diethyl-p-phenylenediamine monohydrochloride.
• Reaction involving selenium, potassium iodide, and the reagent in a low acidic medium.
• Measurement of absorbance at 552 nm.

Main Results:

• The method exhibits a molar extinction coefficient of 6.1 × 10(4) L mol(-1) cm(-1).
• Validated linearity in the range of 0.5-3.0 μg/mL.
• Achieved a limit of detection of 0.0573 μg/mL and a limit of quantification of 0.1737 μg/mL.
• Demonstrated good repeatability, precision, and accuracy.

Conclusions:

• The developed spectrophotometric method is reliable for Se(IV) determination.
• The method was successfully applied to analyze spring water, bottled water, pharmaceutical, and cosmetic products.
• This assay provides a valuable tool for selenium analysis in diverse matrices.