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Published on: May 31, 2018
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Dendritic cells pulsed with leukemia cell-derived exosomes more efficiently induce antileukemic immunities
1Department of Hematology, Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine, Shanghai, China.
Plos One
|March 14, 2014
Summary
Leukemia cell-derived exosomes (LEXs), when used to prime dendritic cells (DCs), effectively induce an antileukemic immune response. This suggests LEX-based vaccines could improve outcomes for leukemia patients.
Area of Science:
- Immunology
- Oncology
- Cell Biology
Background:
- Dendritic cells (DCs) and tumor exosomes are used in antitumor vaccines.
- Biological properties and antileukemic effects of leukemia cell-derived exosomes (LEXs) are not well understood.
Purpose of the Study:
- To investigate the biological properties of LEXs.
- To evaluate the potential of LEXs in inducing antileukemic immunity.
Main Methods:
- Transmission electron microscopy
- Western blot analysis
- Cytotoxicity assays
- Animal studies
- LEXs pulsed DCs
Main Results:
- Leukemia cells release exosomes (LEXs) similar to other tumor cells.
- LEXs contain adhesion and immunological molecules.
- LEXs-pulsed DCs induced a more effective antileukemic cytotoxic T-lymphocyte response and immunity than LEXs or non-pulsed DCs.
Conclusions:
- LEXs harbor leukemia-associated antigens and immunological molecules.
- LEX-based vaccines show promise for prolonging disease-free survival in leukemia patients post-treatment.
