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Related Concept Videos

Translational Regulation01:29

Translational Regulation

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Translational regulation in prokaryotes ensures efficient protein synthesis by controlling ribosome access to mRNA. This regulation is mediated by secondary RNA structures, including translational riboswitches, RNA thermometers, and small RNAs (sRNAs), which respond to intracellular and environmental signals to modulate gene expression.Translational RiboswitchesRiboswitches in the leader region of mRNAs can regulate translation by altering the accessibility of the Shine-Dalgarno (SD) sequence,...
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Cotranslational Protein Translocation01:20

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Translocation of proteins across membranes is an ancient process that occurs even in bacteria and archaebacteria. In fact, the components of the translocation machinery are still conserved between prokaryotes and eukaryotes.
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Within a biological system, the DNA encodes the RNA, and the nucleotide sequence in the RNA further defines the amino acid sequence in the protein. This is referred to as “The Central Dogma of Molecular Biology” - a term coined by Francis Crick.  Central dogma is a firm principle in biology that defines the flow of genetic information within any life form. The two fundamental steps in central dogma are - transcription and translation.
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During most eukaryotic translation processes, the small 40S ribosome subunit scans an mRNA from its 5' end until it encounters the first start AUG codon. The large 60S ribosomal subunit then joins the smaller one to initiate protein synthesis. The location of the translation initiation is largely determined by the nucleotides near the start codon as there may be multiple translation initiation sites present on the mRNA.  Marilyn Kozak discovered that the sequence RCCAUGG (where R...
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Ribosome profiling or ribo-sequencing is a deep sequencing technique that produces a snapshot of active translation in a cell. It selectively sequences the mRNAs protected by ribosomes to get an insight into a cell’s translation landscape at any given point in time.
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Translational research: from pot to plot.

Hilde Nelissen1, Maurice Moloney, Dirk Inzé

  • 1Department of Plant Systems Biology, VIB, Gent, Belgium; Department of Plant Biotechnology and Bioinformatics, Ghent University, Gent, Belgium.

Plant Biotechnology Journal
|March 21, 2014
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Summary
This summary is machine-generated.

Plant scientists must translate basic research into crop applications for food security. This requires field trials and addressing regulatory hurdles for biotech crops.

Keywords:
cropsfield trialsplant biotechnologytranslational research

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Area of Science:

  • Plant molecular biology
  • Translational research in agriculture

Background:

  • Decades of plant molecular biology research have yielded extensive data on plant growth, development, and stress responses.
  • Translating this knowledge into tangible crop improvement for food security and sustainable agriculture remains a significant challenge.

Purpose of the Study:

  • To advocate for increased involvement of plant scientists in converting fundamental biological insights into practical crop applications.
  • To highlight the need for translational research that bridges the gap between model species and crop genetics.
  • To emphasize the importance of field-based evaluation for robust trait development.

Main Methods:

  • Leveraging advanced genome-wide technologies and phenotyping techniques like canopy imaging and field monitoring.
  • Conducting holistic trait assessments under diverse environmental conditions, moving beyond controlled growth chambers.
  • Addressing legislative barriers to the adoption of biotechnology in crops.

Main Results:

  • The study argues that current research translation is insufficient for crop improvement.
  • It identifies poor translatability from model species to crops and the need for genotype-environment interaction understanding.
  • Progress is hindered by regulatory challenges, particularly in Europe.

Conclusions:

  • Plant scientists need to actively engage in translational research to support food security.
  • A network of protected European field trial sites is proposed to facilitate the evaluation of biotech crop traits.
  • Overcoming regulatory obstacles is crucial for advancing crop improvement.