[A kinetic method of determining creatinine using the Jaffé reaction]

The authors suggest a kinetic method for measuring the blood serum and urine creatinine by Jaffe's test. The method is highly specific, requires little biologic material, and rules out the deproteinization procedure. The specificity of the measurement is effected by selecting the period of time when it is creatinine that reacts with alkaline picrate. A thorough selection of the reaction mixture components and of the order of their addition helps minimize the interference of the substances reacting with alkaline picrate during the same period. The method is adapted to biochemical analyzers.